Chromosomal locations of four minor rDNA loci and a marker microsatellite sequence in barley

TY - JOUR

T1 - Chromosomal locations of four minor rDNA loci and a marker microsatellite sequence in barley

AU - Pedersen, Carsten

AU - Linde-Laursen, Ib

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Four minor rDNA loci have been mapped physically to barley (Hordeum vulgare L.) chromosomes 1 (7I), 2 (2I), 4 (4I), and 5 (1I) by a two-step in situ hybridization procedure including a GAA microsatellite sequence. Reprobing with the microsatellite resulted in a distinct banding pattern, resembling the C-banding pattern, which enabled unequivocal chromosome identification. This study suggests that gene mapping accuracy may be improved by using probes with well-characterized and narrow hybridization sites as cytological markers which are situated close to the gene locus. One of the rDNA loci is located about 54% out on the short arm of chromosome 4 and it has not previously been reported in barley. We have disignated the new locus Nor-16. rDNA loci on homoeologous group 4 chromosomes have not yet been reported in other Triticeae species. The origin of these 4 minor rDNA loci is discussed in relation to their equilocal distribution on the chromosomes.

AB - Four minor rDNA loci have been mapped physically to barley (Hordeum vulgare L.) chromosomes 1 (7I), 2 (2I), 4 (4I), and 5 (1I) by a two-step in situ hybridization procedure including a GAA microsatellite sequence. Reprobing with the microsatellite resulted in a distinct banding pattern, resembling the C-banding pattern, which enabled unequivocal chromosome identification. This study suggests that gene mapping accuracy may be improved by using probes with well-characterized and narrow hybridization sites as cytological markers which are situated close to the gene locus. One of the rDNA loci is located about 54% out on the short arm of chromosome 4 and it has not previously been reported in barley. We have disignated the new locus Nor-16. rDNA loci on homoeologous group 4 chromosomes have not yet been reported in other Triticeae species. The origin of these 4 minor rDNA loci is discussed in relation to their equilocal distribution on the chromosomes.

KW - C-banding

KW - chromosome identification

KW - Hordeum vulgare

KW - in situ hybridization

KW - microsatellite

UR - http://www.scopus.com/inward/record.url?scp=0028121052&partnerID=8YFLogxK

U2 - 10.1007/BF01539456

DO - 10.1007/BF01539456

M3 - Journal article

C2 - 8162323

AN - SCOPUS:0028121052

SN - 0967-3849

VL - 2

SP - 65

EP - 71

JO - Chromosome Research

JF - Chromosome Research

IS - 1

ER -