@article{38339f3074c811dbbee902004c4f4f50,
title = "Chromatin-mediated transcriptional regulation by the yeast architectural factors NHP6A and NHP6B",
abstract = "The Saccharomyces cerevisiae NHP6A and NHP6B proteins are chromatin architectural factors, functionally and structurally related to the mammalian high mobility group (HMG)-1 and -2 proteins, a family of non-sequence-specific DNA binding proteins. nhp6a nhp6b mutants have various morphological defects and are defective in the induced expression of several RNA polymerase II-transcribed genes. We found that NHP6A/B proteins are also required for full induction of the yeast CHA1 gene. Importantly, CHA1 basal level expression is increased 10-fold in an nhp6a nhp6b double deletion mutant. Micrococcal nuclease and DNase I analysis of the CHA1 gene in this strain showed an open promoter structure, characteristic of the activated state of this promoter, even under non-inducing conditions. To address the possible function of the NHP6A/B proteins in chromatin-mediated gene regulation, we performed whole-genome transcriptional profiling of a Deltanhp6a Deltanhp6b yeast strain. Our results suggest that NHP6A/B proteins play an important regulatory role, repressing as well as potentiating expression of genes involved in several cellular processes, and that NHP6A/B control is exerted at the level of the individual gene.",
author = "Moreira, {Jos{\'e} Manuel Alfonso} and S Holmberg",
note = "Keywords: Chromatin; DNA-Binding Proteins; Fungal Proteins; Gene Deletion; Gene Expression Regulation, Fungal; Genome, Fungal; Membrane Transport Proteins; Mutation; Nuclear Proteins; Phosphate Transport Proteins; RNA Polymerase II; Repressor Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Transcription, Genetic",
year = "2000",
doi = "10.1093/emboj/19.24.6804",
language = "English",
volume = "19",
pages = "6804--6813",
journal = "E M B O Journal",
issn = "0261-4189",
publisher = "Wiley-Blackwell",
number = "24",
}