Chitosan-dextran sulfate hydrogels as a potential carrier for probiotics

Cigdem Yucel Falco; Peter Falkman; Jens Risbo; Marité Cárdenas; Bruno Medronho

doi:10.1016/j.carbpol.2017.04.047

Chitosan-dextran sulfate hydrogels as a potential carrier for probiotics

Cigdem Yucel Falco, Peter Falkman, Jens Risbo^*, Marité Cárdenas, Bruno Medronho

^*Corresponding author af dette arbejde

25 Citationer (Scopus)

Abstract

Physical and chemical (crosslinked with genipin) hydrogels based on chitosan and dextran sulfate were developed and characterized as novel bio-materials suitable for probiotic encapsulation. The swelling of the hydrogels was dependent on the composition and weakly influenced by the pH of the media. The morphology analysis supports the swelling data showing distinct changes in microstructure depending on the composition. The viability and culturability tests showed approx. 3.6 log CFU/mL decrease of cells (L. acidophilus as model) incorporated into chemical hydrogels when compared to the number of viable native cells. However, the live/dead viability assay evidenced that a considerable amount of viable cells were still entrapped in the hydrogel network and therefore the viability is most likely underestimated. Overall, the developed systems are robust and their structure, rheology and swelling properties can be tuned by changing the blend ratio, thus constituting appealing bio-matrices for cell encapsulation.

Originalsprog	Engelsk
Tidsskrift	Carbohydrate Polymers
Vol/bind	172
Sider (fra-til)	175-183
Antal sider	9
ISSN	0144-8617
DOI	https://doi.org/10.1016/j.carbpol.2017.04.047
Status	Udgivet - 2017

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10.1016/j.carbpol.2017.04.047

Citationsformater

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title = "Chitosan-dextran sulfate hydrogels as a potential carrier for probiotics",

abstract = "Physical and chemical (crosslinked with genipin) hydrogels based on chitosan and dextran sulfate were developed and characterized as novel bio-materials suitable for probiotic encapsulation. The swelling of the hydrogels was dependent on the composition and weakly influenced by the pH of the media. The morphology analysis supports the swelling data showing distinct changes in microstructure depending on the composition. The viability and culturability tests showed approx. 3.6 log CFU/mL decrease of cells (L. acidophilus as model) incorporated into chemical hydrogels when compared to the number of viable native cells. However, the live/dead viability assay evidenced that a considerable amount of viable cells were still entrapped in the hydrogel network and therefore the viability is most likely underestimated. Overall, the developed systems are robust and their structure, rheology and swelling properties can be tuned by changing the blend ratio, thus constituting appealing bio-matrices for cell encapsulation.",

keywords = "Chitosan, Dextran sulfate, Encapsulation, Genipin, Hydrogel, Probiotic bacteria",

author = "{Yucel Falco}, Cigdem and Peter Falkman and Jens Risbo and Marit{\'e} C{\'a}rdenas and Bruno Medronho",

year = "2017",

doi = "10.1016/j.carbpol.2017.04.047",

language = "English",

volume = "172",

pages = "175--183",

journal = "Carbohydrate Polymers",

issn = "0144-8617",

publisher = "Pergamon Press",

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TY - JOUR

T1 - Chitosan-dextran sulfate hydrogels as a potential carrier for probiotics

AU - Yucel Falco, Cigdem

AU - Falkman, Peter

AU - Risbo, Jens

AU - Cárdenas, Marité

AU - Medronho, Bruno

PY - 2017

Y1 - 2017

N2 - Physical and chemical (crosslinked with genipin) hydrogels based on chitosan and dextran sulfate were developed and characterized as novel bio-materials suitable for probiotic encapsulation. The swelling of the hydrogels was dependent on the composition and weakly influenced by the pH of the media. The morphology analysis supports the swelling data showing distinct changes in microstructure depending on the composition. The viability and culturability tests showed approx. 3.6 log CFU/mL decrease of cells (L. acidophilus as model) incorporated into chemical hydrogels when compared to the number of viable native cells. However, the live/dead viability assay evidenced that a considerable amount of viable cells were still entrapped in the hydrogel network and therefore the viability is most likely underestimated. Overall, the developed systems are robust and their structure, rheology and swelling properties can be tuned by changing the blend ratio, thus constituting appealing bio-matrices for cell encapsulation.

AB - Physical and chemical (crosslinked with genipin) hydrogels based on chitosan and dextran sulfate were developed and characterized as novel bio-materials suitable for probiotic encapsulation. The swelling of the hydrogels was dependent on the composition and weakly influenced by the pH of the media. The morphology analysis supports the swelling data showing distinct changes in microstructure depending on the composition. The viability and culturability tests showed approx. 3.6 log CFU/mL decrease of cells (L. acidophilus as model) incorporated into chemical hydrogels when compared to the number of viable native cells. However, the live/dead viability assay evidenced that a considerable amount of viable cells were still entrapped in the hydrogel network and therefore the viability is most likely underestimated. Overall, the developed systems are robust and their structure, rheology and swelling properties can be tuned by changing the blend ratio, thus constituting appealing bio-matrices for cell encapsulation.

KW - Chitosan

KW - Dextran sulfate

KW - Encapsulation

KW - Genipin

KW - Hydrogel

KW - Probiotic bacteria

U2 - 10.1016/j.carbpol.2017.04.047

DO - 10.1016/j.carbpol.2017.04.047

M3 - Journal article

C2 - 28606523

AN - SCOPUS:85019562805

SN - 0144-8617

VL - 172

SP - 175

EP - 183

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

ER -

Chitosan-dextran sulfate hydrogels as a potential carrier for probiotics

Abstract

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