Characterization of vancomycin-resistant and vancomycin-susceptible Enterococcus faecium isolates from humans, chickens and pigs by RiboPrinting and pulsed-field gel electrophoresis

A M Hammerum; V Fussing; F M Aarestrup; Henrik Caspar Wegener

Characterization of vancomycin-resistant and vancomycin-susceptible Enterococcus faecium isolates from humans, chickens and pigs by RiboPrinting and pulsed-field gel electrophoresis

A M Hammerum, V Fussing, F M Aarestrup, Henrik Caspar Wegener

38 Citationer (Scopus)

Abstract

Forty-eight vancomycin-resistant and 35 vancomycin-sensitive Danish Enterococcus faecium isolates obtained from pigs, chickens and humans, as well as the human vanA reference isolate BM4147, were characterized by EcoRI RiboPrinting and SmaI pulsed-field gel electrophoresis. RiboPrinting of the 84 isolates yielded 40 types whereas PFGE-typing yielded 57 types discriminated by differences in more than three bands. By molecular typing, both clonal spread of E. faecium as well as horizontal transmission of Tn1546 between animals and humans was supported. Furthermore, it was found that the population of E. faecium spreads freely between the animal and human reservoir.

Originalsprog	Engelsk
Tidsskrift	Journal of Antimicrobial Chemotherapy
Vol/bind	45
Udgave nummer	5
Sider (fra-til)	677-80
Antal sider	4
ISSN	0305-7453
Status	Udgivet - maj 2000
Udgivet eksternt	Ja

Citationsformater

Characterization of vancomycin-resistant and vancomycin-susceptible Enterococcus faecium isolates from humans, chickens and pigs by RiboPrinting and pulsed-field gel electrophoresis. / Hammerum, A M; Fussing, V; Aarestrup, F M et al.

I: Journal of Antimicrobial Chemotherapy, Bind 45, Nr. 5, 05.2000, s. 677-80.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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title = "Characterization of vancomycin-resistant and vancomycin-susceptible Enterococcus faecium isolates from humans, chickens and pigs by RiboPrinting and pulsed-field gel electrophoresis",

abstract = "Forty-eight vancomycin-resistant and 35 vancomycin-sensitive Danish Enterococcus faecium isolates obtained from pigs, chickens and humans, as well as the human vanA reference isolate BM4147, were characterized by EcoRI RiboPrinting and SmaI pulsed-field gel electrophoresis. RiboPrinting of the 84 isolates yielded 40 types whereas PFGE-typing yielded 57 types discriminated by differences in more than three bands. By molecular typing, both clonal spread of E. faecium as well as horizontal transmission of Tn1546 between animals and humans was supported. Furthermore, it was found that the population of E. faecium spreads freely between the animal and human reservoir.",

keywords = "Animals, Anti-Bacterial Agents, Bacterial Proteins, Bacterial Typing Techniques, Carbon-Oxygen Ligases, Chickens, Deoxyribonuclease EcoRI, Deoxyribonucleases, Type II Site-Specific, Electrophoresis, Gel, Pulsed-Field, Enterococcus faecium, Gram-Positive Bacterial Infections, Humans, Poultry Diseases, Restriction Mapping, Swine, Swine Diseases, Vancomycin, Vancomycin Resistance, Journal Article, Research Support, Non-U.S. Gov't",

author = "Hammerum, {A M} and V Fussing and Aarestrup, {F M} and Wegener, {Henrik Caspar}",

year = "2000",

month = may,

language = "English",

volume = "45",

pages = "677--80",

journal = "Journal of Antimicrobial Chemotherapy",

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TY - JOUR

T1 - Characterization of vancomycin-resistant and vancomycin-susceptible Enterococcus faecium isolates from humans, chickens and pigs by RiboPrinting and pulsed-field gel electrophoresis

AU - Hammerum, A M

AU - Fussing, V

AU - Aarestrup, F M

AU - Wegener, Henrik Caspar

PY - 2000/5

Y1 - 2000/5

N2 - Forty-eight vancomycin-resistant and 35 vancomycin-sensitive Danish Enterococcus faecium isolates obtained from pigs, chickens and humans, as well as the human vanA reference isolate BM4147, were characterized by EcoRI RiboPrinting and SmaI pulsed-field gel electrophoresis. RiboPrinting of the 84 isolates yielded 40 types whereas PFGE-typing yielded 57 types discriminated by differences in more than three bands. By molecular typing, both clonal spread of E. faecium as well as horizontal transmission of Tn1546 between animals and humans was supported. Furthermore, it was found that the population of E. faecium spreads freely between the animal and human reservoir.

AB - Forty-eight vancomycin-resistant and 35 vancomycin-sensitive Danish Enterococcus faecium isolates obtained from pigs, chickens and humans, as well as the human vanA reference isolate BM4147, were characterized by EcoRI RiboPrinting and SmaI pulsed-field gel electrophoresis. RiboPrinting of the 84 isolates yielded 40 types whereas PFGE-typing yielded 57 types discriminated by differences in more than three bands. By molecular typing, both clonal spread of E. faecium as well as horizontal transmission of Tn1546 between animals and humans was supported. Furthermore, it was found that the population of E. faecium spreads freely between the animal and human reservoir.

KW - Animals

KW - Anti-Bacterial Agents

KW - Bacterial Proteins

KW - Bacterial Typing Techniques

KW - Carbon-Oxygen Ligases

KW - Chickens

KW - Deoxyribonuclease EcoRI

KW - Deoxyribonucleases, Type II Site-Specific

KW - Electrophoresis, Gel, Pulsed-Field

KW - Enterococcus faecium

KW - Gram-Positive Bacterial Infections

KW - Humans

KW - Poultry Diseases

KW - Restriction Mapping

KW - Swine

KW - Swine Diseases

KW - Vancomycin

KW - Vancomycin Resistance

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Journal article

C2 - 10797092

SN - 0305-7453

VL - 45

SP - 677

EP - 680

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

IS - 5

ER -

Characterization of vancomycin-resistant and vancomycin-susceptible Enterococcus faecium isolates from humans, chickens and pigs by RiboPrinting and pulsed-field gel electrophoresis

Abstract

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Citationsformater