Characterization of microsatellite markers isolated from the American Mink (Mustela vison) genome

S. Ansari; Razvan Marian Anistoroaei; A. Farid; Knud Christensen; B.F. Benkel

Characterization of microsatellite markers isolated from the American Mink (Mustela vison) genome

S. Ansari, Razvan Marian Anistoroaei, A. Farid, Knud Christensen, B.F. Benkel

Abstract

Two size-selected mink genomic libraries were constructed and recombinant colonies (n=6,144) were screened with a pool of probes, containing (AAAG)₈, (AAGG)₈, (AGGG)_8,(ATAG)₈ and (AG)₁₅oligonucleotides in equal amounts. A total of 44 colonies were hybridized and confirmed upon replating. Sequencing of the clones revealed 35 microsatellites with unique sequences. There were 19 di- and 6 tetra nucleotides, and the remaining 10 had complex repeats. Twenty eight (80%) were polymorphic in a panel of 20 mink from two Danish Farms.

Originalsprog	Engelsk
Tidsskrift	Scientifur
Vol/bind	31
Udgave nummer	2
Sider (fra-til)	55-58
Antal sider	4
ISSN	0105-2403
Status	Udgivet - 2007

Emneord

Det tidligere LIFE

Citationsformater

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title = "Characterization of microsatellite markers isolated from the American Mink (Mustela vison) genome",

abstract = "Two size-selected mink genomic libraries were constructed and recombinant colonies (n=6,144) were screened with a pool of probes, containing (AAAG)8, (AAGG)8, (AGGG)8, (ATAG)8 and (AG)15 oligonucleotides in equal amounts. A total of 44 colonies were hybridized and confirmed upon replating. Sequencing of the clones revealed 35 microsatellites with unique sequences. There were 19 di- and 6 tetra nucleotides, and the remaining 10 had complex repeats. Twenty eight (80%) were polymorphic in a panel of 20 mink from two Danish Farms.",

keywords = "Former LIFE faculty, American mink, microsattelite, polymorphism",

author = "S. Ansari and Anistoroaei, {Razvan Marian} and A. Farid and Knud Christensen and B.F. Benkel",

year = "2007",

language = "English",

volume = "31",

pages = "55--58",

journal = "Scientifur",

issn = "0105-2403",

publisher = "International Fur Animal Scientific Association",

number = "2",

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TY - JOUR

T1 - Characterization of microsatellite markers isolated from the American Mink (Mustela vison) genome

AU - Ansari, S.

AU - Anistoroaei, Razvan Marian

AU - Farid, A.

AU - Christensen, Knud

AU - Benkel, B.F.

PY - 2007

Y1 - 2007

N2 - Two size-selected mink genomic libraries were constructed and recombinant colonies (n=6,144) were screened with a pool of probes, containing (AAAG)8, (AAGG)8, (AGGG)8, (ATAG)8 and (AG)15 oligonucleotides in equal amounts. A total of 44 colonies were hybridized and confirmed upon replating. Sequencing of the clones revealed 35 microsatellites with unique sequences. There were 19 di- and 6 tetra nucleotides, and the remaining 10 had complex repeats. Twenty eight (80%) were polymorphic in a panel of 20 mink from two Danish Farms.

AB - Two size-selected mink genomic libraries were constructed and recombinant colonies (n=6,144) were screened with a pool of probes, containing (AAAG)8, (AAGG)8, (AGGG)8, (ATAG)8 and (AG)15 oligonucleotides in equal amounts. A total of 44 colonies were hybridized and confirmed upon replating. Sequencing of the clones revealed 35 microsatellites with unique sequences. There were 19 di- and 6 tetra nucleotides, and the remaining 10 had complex repeats. Twenty eight (80%) were polymorphic in a panel of 20 mink from two Danish Farms.

KW - Former LIFE faculty

KW - American mink

KW - microsattelite

KW - polymorphism

M3 - Journal article

SN - 0105-2403

VL - 31

SP - 55

EP - 58

JO - Scientifur

JF - Scientifur

IS - 2

ER -

Characterization of microsatellite markers isolated from the American Mink (Mustela vison) genome

Abstract

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Fingeraftryk

Citationsformater