Characterization of ex vivo expanded tumor infiltrating lymphocytes from patients with malignant melanoma for clinical application

Niels Junker; Per Thor Straten; Mads Hald Andersen; Inge Marie Svane

doi:10.1155/2011/574695

Characterization of ex vivo expanded tumor infiltrating lymphocytes from patients with malignant melanoma for clinical application

Niels Junker, Per Thor Straten, Mads Hald Andersen, Inge Marie Svane

Abstract

Clinical trials of adoptive transfer of autologous tumor infiltrating lymphocytes (TILs) to patients with advanced malignant melanoma have shown remarkable results with objective clinical responses in 50% of the treated patients. In order to initiate a clinical trial in melanoma, we have established a method for expanding TILs to clinical relevant quantities in two steps with in 8 weeks. Further characterization of expanded TILs revealed an oligoclonal composition of T-cells with an effector memory like phenotype. When autologous tumor was available, TILs showed specific activity in all patients tested. TIL cultures contained specificity towards tumor cells as well as peptides derived from tumor-associated antigens (TAAs) during expansion procedures.

Originalsprog	Engelsk
Tidsskrift	Journal of Skin Cancer
Vol/bind	2011
Udgave nummer	574695
Antal sider	6
ISSN	2090-2905
DOI	https://doi.org/10.1155/2011/574695
Status	Udgivet - 2011

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10.1155/2011/574695

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title = "Characterization of ex vivo expanded tumor infiltrating lymphocytes from patients with malignant melanoma for clinical application",

abstract = "Clinical trials of adoptive transfer of autologous tumor infiltrating lymphocytes (TILs) to patients with advanced malignant melanoma have shown remarkable results with objective clinical responses in 50% of the treated patients. In order to initiate a clinical trial in melanoma, we have established a method for expanding TILs to clinical relevant quantities in two steps with in 8 weeks. Further characterization of expanded TILs revealed an oligoclonal composition of T-cells with an effector memory like phenotype. When autologous tumor was available, TILs showed specific activity in all patients tested. TIL cultures contained specificity towards tumor cells as well as peptides derived from tumor-associated antigens (TAAs) during expansion procedures.",

author = "Niels Junker and {Thor Straten}, Per and Andersen, {Mads Hald} and Svane, {Inge Marie}",

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doi = "10.1155/2011/574695",

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T1 - Characterization of ex vivo expanded tumor infiltrating lymphocytes from patients with malignant melanoma for clinical application

AU - Junker, Niels

AU - Thor Straten, Per

AU - Andersen, Mads Hald

AU - Svane, Inge Marie

PY - 2011

Y1 - 2011

N2 - Clinical trials of adoptive transfer of autologous tumor infiltrating lymphocytes (TILs) to patients with advanced malignant melanoma have shown remarkable results with objective clinical responses in 50% of the treated patients. In order to initiate a clinical trial in melanoma, we have established a method for expanding TILs to clinical relevant quantities in two steps with in 8 weeks. Further characterization of expanded TILs revealed an oligoclonal composition of T-cells with an effector memory like phenotype. When autologous tumor was available, TILs showed specific activity in all patients tested. TIL cultures contained specificity towards tumor cells as well as peptides derived from tumor-associated antigens (TAAs) during expansion procedures.

AB - Clinical trials of adoptive transfer of autologous tumor infiltrating lymphocytes (TILs) to patients with advanced malignant melanoma have shown remarkable results with objective clinical responses in 50% of the treated patients. In order to initiate a clinical trial in melanoma, we have established a method for expanding TILs to clinical relevant quantities in two steps with in 8 weeks. Further characterization of expanded TILs revealed an oligoclonal composition of T-cells with an effector memory like phenotype. When autologous tumor was available, TILs showed specific activity in all patients tested. TIL cultures contained specificity towards tumor cells as well as peptides derived from tumor-associated antigens (TAAs) during expansion procedures.

U2 - 10.1155/2011/574695

DO - 10.1155/2011/574695

M3 - Journal article

SN - 2090-2905

VL - 2011

JO - Journal of Skin Cancer

JF - Journal of Skin Cancer

IS - 574695

ER -

Characterization of ex vivo expanded tumor infiltrating lymphocytes from patients with malignant melanoma for clinical application

Abstract

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