Central Metabolic Responses to Ozone and Herbivory Affect Photosynthesis and Stomatal Closure

TY - JOUR

T1 - Central Metabolic Responses to Ozone and Herbivory Affect Photosynthesis and Stomatal Closure

AU - Papazian, Stefano

AU - Khaling, Eliezer

AU - Bonnet, Christelle

AU - Lassueur, Steve

AU - Reymond, Philippe

AU - Moritz, Thomas

AU - Blande, James D.

AU - Albrectsen, Benedicte R.

PY - 2016/11

Y1 - 2016/11

N2 - Plants have evolved adaptive mechanisms that allow them to tolerate a continuous range of abiotic and biotic stressors. Tropospheric ozone (O3), a global anthropogenic pollutant, directly affects living organisms and ecosystems, including plant-herbivore interactions. In this study, we investigate the stress responses of Brassica nigra (wild black mustard) exposed consecutively to O3and the specialist herbivore Pieris brassicae. Transcriptomics and metabolomics data were evaluated using multivariate, correlation, and network analyses for the O3and herbivory responses. O3stress symptoms resembled those of senescence and phosphate starvation, while a sequential shift from O3to herbivory induced characteristic plant defense responses, including a decrease in central metabolism, induction of the jasmonic acid/ethylene pathways, and emission of volatiles. Omics network and pathway analyses predicted a link between glycerol and central energy metabolism that influences the osmotic stress response and stomatal closure. Further physiological measurements confirmed that while O3stress inhibited photosynthesis and carbon assimilation, sequential herbivory counteracted the initial responses induced by O3, resulting in a phenotype similar to that observed after herbivory alone. This study clarifies the consequences of multiple stress interactions on a plant metabolic system and also illustrates how omics data can be integrated to generate new hypotheses in ecology and plant physiology.

AB - Plants have evolved adaptive mechanisms that allow them to tolerate a continuous range of abiotic and biotic stressors. Tropospheric ozone (O3), a global anthropogenic pollutant, directly affects living organisms and ecosystems, including plant-herbivore interactions. In this study, we investigate the stress responses of Brassica nigra (wild black mustard) exposed consecutively to O3and the specialist herbivore Pieris brassicae. Transcriptomics and metabolomics data were evaluated using multivariate, correlation, and network analyses for the O3and herbivory responses. O3stress symptoms resembled those of senescence and phosphate starvation, while a sequential shift from O3to herbivory induced characteristic plant defense responses, including a decrease in central metabolism, induction of the jasmonic acid/ethylene pathways, and emission of volatiles. Omics network and pathway analyses predicted a link between glycerol and central energy metabolism that influences the osmotic stress response and stomatal closure. Further physiological measurements confirmed that while O3stress inhibited photosynthesis and carbon assimilation, sequential herbivory counteracted the initial responses induced by O3, resulting in a phenotype similar to that observed after herbivory alone. This study clarifies the consequences of multiple stress interactions on a plant metabolic system and also illustrates how omics data can be integrated to generate new hypotheses in ecology and plant physiology.

U2 - 10.1104/pp.16.01318

DO - 10.1104/pp.16.01318

M3 - Journal article

SN - 0032-0889

VL - 172

SP - 2057

EP - 2078

JO - Plant Physiology

JF - Plant Physiology

IS - 3

ER -