Cellular endocytic compartment localization of expressed canine CD1 molecules

Mette Schjærff; Stefan M. Keller; Verena K. Affolter; Annemarie Thuri Kristensen; Peter F. Moore

doi:10.1016/j.vetimm.2016.08.012

Cellular endocytic compartment localization of expressed canine CD1 molecules

Mette Schjærff, Stefan M. Keller, Verena K. Affolter, Annemarie Thuri Kristensen, Peter F. Moore

Abstract

CD1 molecules are glycoproteins present primarily on dendritic cells (DCs), which recognize and presenta variety of foreign- and self-lipid antigens to T-cells. Humans have five different CD1 isoforms that sur-vey distinct cellular compartments allowing for recognition of a large repertoire of lipids. The canine CD1family consists of seven functional CD1 molecules (canine CD1a2, CD1a6, CD1a8, CD1a9, CD1b, CD1c andCD1e) and one presumed non-functional isoform (canine CD1d) due to a disrupted gene structure. Theaim of this study was to describe in vitro steady-state localization ptterns of canine CD1 isoforms andtheir correlation with endocytic organelles. GFP-fused canine CD1 293T cell transfectants were stainedwith markers for early endocytic compartments (EEA-1) and late endocytic/lysosomal compartments (LAMP-1), respectively, and analyzed by confocal microscopy. Canine CD1a molecules localized to theplasma membrane and partially to the early endocytic compartment, but not to late endosomes or lyso-somes. In contrast, canine CD1b was highly associated with late endosomal/lysosomal compartments andshowed a predominant intracellular expression pattern. Canine CD1c protein expression localized morepromiscuously to both the early endosomal compartments and the late endosomal/lysosomal compart-ments. The canine CD1e molecule showed a strictly intracellular expression with a partial overlap withlate endosomal/lysosomal compartments. Lastly, canine CD1d was expressed abnormally showing onlya diminished GFP expression. In conclusion, canine CD1 transfectants show distinct localization patternsthat are similar to human CD1 proteins with the exception of the canine CD1d isoform, which most likelyis non-functional. These findings imply that canine CD1 localization overall resembles human CD1 traf-ficking patterns. This knowledge is important for the understanding of lipid antigen-receptor immunityin the dog.

Originalsprog	Engelsk
Tidsskrift	Veterinary Immunology and Immunopathology
Vol/bind	182
Sider (fra-til)	11-21
Antal sider	11
ISSN	0165-2427
DOI	https://doi.org/10.1016/j.vetimm.2016.08.012
Status	Udgivet - 1 dec. 2016

Adgang til dokumentet

10.1016/j.vetimm.2016.08.012

Citationsformater

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title = "Cellular endocytic compartment localization of expressed canine CD1 molecules",

abstract = "CD1 molecules are glycoproteins present primarily on dendritic cells (DCs), which recognize and presenta variety of foreign- and self-lipid antigens to T-cells. Humans have five different CD1 isoforms that sur-vey distinct cellular compartments allowing for recognition of a large repertoire of lipids. The canine CD1family consists of seven functional CD1 molecules (canine CD1a2, CD1a6, CD1a8, CD1a9, CD1b, CD1c andCD1e) and one presumed non-functional isoform (canine CD1d) due to a disrupted gene structure. Theaim of this study was to describe in vitro steady-state localization ptterns of canine CD1 isoforms andtheir correlation with endocytic organelles. GFP-fused canine CD1 293T cell transfectants were stainedwith markers for early endocytic compartments (EEA-1) and late endocytic/lysosomal compartments (LAMP-1), respectively, and analyzed by confocal microscopy. Canine CD1a molecules localized to theplasma membrane and partially to the early endocytic compartment, but not to late endosomes or lyso-somes. In contrast, canine CD1b was highly associated with late endosomal/lysosomal compartments andshowed a predominant intracellular expression pattern. Canine CD1c protein expression localized morepromiscuously to both the early endosomal compartments and the late endosomal/lysosomal compart-ments. The canine CD1e molecule showed a strictly intracellular expression with a partial overlap withlate endosomal/lysosomal compartments. Lastly, canine CD1d was expressed abnormally showing onlya diminished GFP expression. In conclusion, canine CD1 transfectants show distinct localization patternsthat are similar to human CD1 proteins with the exception of the canine CD1d isoform, which most likelyis non-functional. These findings imply that canine CD1 localization overall resembles human CD1 traf-ficking patterns. This knowledge is important for the understanding of lipid antigen-receptor immunityin the dog.",

keywords = "Canine CD1 molecules, Canine CD1 intracellular localization, Endocytic compartments, Cytoplasmic tail trafficking motif, Confocal microscopy",

author = "Mette Schj{\ae}rff and Keller, {Stefan M.} and Affolter, {Verena K.} and Kristensen, {Annemarie Thuri} and Moore, {Peter F.}",

year = "2016",

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doi = "10.1016/j.vetimm.2016.08.012",

language = "English",

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journal = "Veterinary Immunology and Immunopathology",

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TY - JOUR

T1 - Cellular endocytic compartment localization of expressed canine CD1 molecules

AU - Schjærff, Mette

AU - Keller, Stefan M.

AU - Affolter, Verena K.

AU - Kristensen, Annemarie Thuri

AU - Moore, Peter F.

PY - 2016/12/1

Y1 - 2016/12/1

N2 - CD1 molecules are glycoproteins present primarily on dendritic cells (DCs), which recognize and presenta variety of foreign- and self-lipid antigens to T-cells. Humans have five different CD1 isoforms that sur-vey distinct cellular compartments allowing for recognition of a large repertoire of lipids. The canine CD1family consists of seven functional CD1 molecules (canine CD1a2, CD1a6, CD1a8, CD1a9, CD1b, CD1c andCD1e) and one presumed non-functional isoform (canine CD1d) due to a disrupted gene structure. Theaim of this study was to describe in vitro steady-state localization ptterns of canine CD1 isoforms andtheir correlation with endocytic organelles. GFP-fused canine CD1 293T cell transfectants were stainedwith markers for early endocytic compartments (EEA-1) and late endocytic/lysosomal compartments (LAMP-1), respectively, and analyzed by confocal microscopy. Canine CD1a molecules localized to theplasma membrane and partially to the early endocytic compartment, but not to late endosomes or lyso-somes. In contrast, canine CD1b was highly associated with late endosomal/lysosomal compartments andshowed a predominant intracellular expression pattern. Canine CD1c protein expression localized morepromiscuously to both the early endosomal compartments and the late endosomal/lysosomal compart-ments. The canine CD1e molecule showed a strictly intracellular expression with a partial overlap withlate endosomal/lysosomal compartments. Lastly, canine CD1d was expressed abnormally showing onlya diminished GFP expression. In conclusion, canine CD1 transfectants show distinct localization patternsthat are similar to human CD1 proteins with the exception of the canine CD1d isoform, which most likelyis non-functional. These findings imply that canine CD1 localization overall resembles human CD1 traf-ficking patterns. This knowledge is important for the understanding of lipid antigen-receptor immunityin the dog.

AB - CD1 molecules are glycoproteins present primarily on dendritic cells (DCs), which recognize and presenta variety of foreign- and self-lipid antigens to T-cells. Humans have five different CD1 isoforms that sur-vey distinct cellular compartments allowing for recognition of a large repertoire of lipids. The canine CD1family consists of seven functional CD1 molecules (canine CD1a2, CD1a6, CD1a8, CD1a9, CD1b, CD1c andCD1e) and one presumed non-functional isoform (canine CD1d) due to a disrupted gene structure. Theaim of this study was to describe in vitro steady-state localization ptterns of canine CD1 isoforms andtheir correlation with endocytic organelles. GFP-fused canine CD1 293T cell transfectants were stainedwith markers for early endocytic compartments (EEA-1) and late endocytic/lysosomal compartments (LAMP-1), respectively, and analyzed by confocal microscopy. Canine CD1a molecules localized to theplasma membrane and partially to the early endocytic compartment, but not to late endosomes or lyso-somes. In contrast, canine CD1b was highly associated with late endosomal/lysosomal compartments andshowed a predominant intracellular expression pattern. Canine CD1c protein expression localized morepromiscuously to both the early endosomal compartments and the late endosomal/lysosomal compart-ments. The canine CD1e molecule showed a strictly intracellular expression with a partial overlap withlate endosomal/lysosomal compartments. Lastly, canine CD1d was expressed abnormally showing onlya diminished GFP expression. In conclusion, canine CD1 transfectants show distinct localization patternsthat are similar to human CD1 proteins with the exception of the canine CD1d isoform, which most likelyis non-functional. These findings imply that canine CD1 localization overall resembles human CD1 traf-ficking patterns. This knowledge is important for the understanding of lipid antigen-receptor immunityin the dog.

KW - Canine CD1 molecules

KW - Canine CD1 intracellular localization

KW - Endocytic compartments

KW - Cytoplasmic tail trafficking motif

KW - Confocal microscopy

U2 - 10.1016/j.vetimm.2016.08.012

DO - 10.1016/j.vetimm.2016.08.012

M3 - Journal article

C2 - 27863541

SN - 0165-2427

VL - 182

SP - 11

EP - 21

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

ER -