Cell-induced potentiation of the plasminogen activation system is abolished by a monoclonal antibody that recognizes the NH2-terminal domain of the urokinase receptor

TY - JOUR

T1 - Cell-induced potentiation of the plasminogen activation system is abolished by a monoclonal antibody that recognizes the NH2-terminal domain of the urokinase receptor

AU - Rønne, E

AU - Behrendt, N

AU - Ellis, V

AU - Ploug, M

AU - Danø, K

AU - Høyer-Hansen, G

PY - 1991/8/19

Y1 - 1991/8/19

N2 - We have raised four monoclonal antibodies recognizing different epitopes within the human cell-surface receptor for urokinase-type plasminogen activator (u-PA). One of these antibodies completely abolishes the potentiation of plasmin generation observed upon incubation of the zymogens pro-u-PA and plasminogen with U937 cells. This antibody, which is also the only one to completely inhibit the binding of DFP-inactivated [125I]-u-PA to U937 cells, is directed against the u-PA binding NH2-terminal domain of u-PAR, a well-defined fragment formed by limited chymotrypsin digestion of purified u-PAR, demonstrating the functional independence of the u-PA binding domain as well as the critical role of u-PAR in the assembly of the cell-surface plasminogen activation system.

AB - We have raised four monoclonal antibodies recognizing different epitopes within the human cell-surface receptor for urokinase-type plasminogen activator (u-PA). One of these antibodies completely abolishes the potentiation of plasmin generation observed upon incubation of the zymogens pro-u-PA and plasminogen with U937 cells. This antibody, which is also the only one to completely inhibit the binding of DFP-inactivated [125I]-u-PA to U937 cells, is directed against the u-PA binding NH2-terminal domain of u-PAR, a well-defined fragment formed by limited chymotrypsin digestion of purified u-PAR, demonstrating the functional independence of the u-PA binding domain as well as the critical role of u-PAR in the assembly of the cell-surface plasminogen activation system.

KW - Antibodies, Monoclonal

KW - Cell Line

KW - Chymotrypsin

KW - Epitopes

KW - Fibrinolysin

KW - Humans

KW - Kinetics

KW - Plasminogen

KW - Plasminogen Activators

KW - Precipitin Tests

KW - Receptors, Cell Surface

KW - Receptors, Urokinase Plasminogen Activator

KW - Structure-Activity Relationship

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Journal article

C2 - 1715292

SN - 0014-5793

VL - 288

SP - 233

EP - 236

JO - F E B S Letters

JF - F E B S Letters

IS - 1-2

ER -