@inbook{15ff1a79717d45f0b7b9dae39db27245,
title = "Cell-based lipid flippase assay employing fluorescent lipid derivatives",
abstract = "P-type ATPases in the P4 subfamily (P4-ATPases) are transmembrane proteins unique for eukaryotes that act as lipid flippases, i.e., to translocate phospholipids from the exofacial to the cytofacial monolayer of cellular membranes. While initially characterized as aminophospholipid translocases, studies of individual P4-ATPase family members from fungi, plants, and animals show that P4-ATPases differ in their substrate specificities and mediate transport of a broader range of lipid substrates. Here, we describe an assay based on fluorescent lipid derivatives to monitor and characterize lipid flippase activities in the plasma membrane of cells, using yeast as an example.",
keywords = "Azoles, Enzyme Assays, Flow Cytometry, Fluorescent Dyes, Lipid Metabolism, Lipids, Nitrobenzenes, Phospholipid Transfer Proteins, Saccharomyces cerevisiae, Journal Article",
author = "Jensen, {Maria Stumph} and Sara Costa and Thomas G{\"u}nther-Pomorski and {Lopez Marques}, {Rosa Laura}",
year = "2016",
month = jan,
day = "1",
doi = "10.1007/978-1-4939-3179-8_33",
language = "English",
isbn = "978-1-4939-3178-1",
volume = "VI",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "371--382",
editor = "Maike Bublitz",
booktitle = "P-Type ATPases",
}