@article{8b5715f05e3911dd8d9f000ea68e967b,
title = "CBFA1 and topoisomerase I mRNA levels decline during cellular aging of human trabecular osteoblasts.",
abstract = "In order to understand the reasons for age-related impairment of the function of bone forming osteoblasts, we have examined the steady-state mRNA levels of the transcription factor CBFA1 and topoisomerase I during cellular aging of normal human trabecular osteoblasts, by the use of semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR). There is a progressive and significant reduction of the CBFA1 steady-state mRNA level down to 50% during cellular aging of human osteoblasts. In comparison to the normal cells, human osteosarcoma cell lines SaOS-2 and KHOS/NP, and the SV40-transformed human lung fibroblast cell line MRC5V2 have 20 to 40% higher levels of CBFA1 mRNA. Similar levels of CBFA1 mRNA are detectable in normal human skin fibroblasts, and these cells also exhibit an age-related decline to the same extent. In addition, the expression of topoisomerase I is reduced by 40% in senescent osteoblasts, and the mRNA levels are significantly higher (40-70%) in transformed osteoblasts and fibroblasts. These changes in gene expression may be among the causes of impaired osteoblast functions, resulting in reduced bone formation during aging.",
author = "M Christiansen and M Kveiborg and M Kassem and Clark, {B F} and Rattan, {S I}",
note = "Keywords: Adult; Animals; Cell Aging; Cell Division; Cells, Cultured; Core Binding Factor Alpha 1 Subunit; DNA Topoisomerases, Type I; Female; Gene Expression; Glyceraldehyde-3-Phosphate Dehydrogenases; Humans; Mice; Neoplasm Proteins; Osteoblasts; RNA, Messenger; Reverse Transcriptase Polymerase Chain Reaction; Transcription Factors; Tumor Cells, Cultured",
year = "2000",
language = "English",
volume = "55",
pages = "B194--200",
journal = "Journals of Gerontology. Series A: Biological Sciences & Medical Sciences",
issn = "1079-5006",
publisher = "Oxford University Press",
number = "4",
}