Cardiac natriuretic peptide gene expression and plasma concentrations during the first 72 hours of life in piglets

Julie Smith; Christina Christoffersen; Linn Maiken Nørgaard; Lisbeth Høier Olsen; Niels G. Vejlstrup; Claus Bøgelund Andersen; Jens P. Gøtze

doi:10.1210/en.2012-2186

Cardiac natriuretic peptide gene expression and plasma concentrations during the first 72 hours of life in piglets

Julie Smith, Christina Christoffersen, Linn Maiken Nørgaard, Lisbeth Høier Olsen, Niels G. Vejlstrup, Claus Bøgelund Andersen, Jens P. Gøtze

Lifepharm

6 Citationer (Scopus)

Abstract

Plasma measurement of cardiac natriuretic peptides constitutes promising markers of congenital heart disease. However, concentrations change rapidly and dramatically during the first days after delivery even in healthy neonates, which complicates clinical interpretation. It is unknown whether these changes in plasma concentrations are explained by corresponding changes in the cardiac gene expression. We quantified the chamber-specific mRNA levels of ANP (A-type natriuretic peptide) and BNP (B-type natriuretic peptide) and plasma pro-ANP and BNP-32 concentrations in healthy piglets during the first 72 hours of life (from 2 litters, n = 44). Chamber-specific ANP and BNP mRNA levels reflected hemodynamic neonate changes at birth but did not correlate with circulating natriuretic peptide concentrations. However, plasma pro-ANP and creatinine concentrations were closely correlated (P < .0001; r = 0.73). Plasma pro-ANP levels were highest on the day of delivery (5580 pmol/L [4320-6786] decreasing to 2484 pmol/L [1602-2898] after 72 hours, P < .0001). During the 72 hours, gel chromatography suggested that the translational products in circulation and in atrial tissue were immature, ie, unprocessed pro-ANP. In contrast to pro-ANP, BNP-32 plasma concentrations were low at delivery and peaked after 48 hours (12 [10.5-20.6] vs. 88.8 [71.7-101.4] pmol/L, P < .0001). To conclude, ANP and BNP gene expression differs considerably between cardiac chambers in the first 72 hours of life in healthy piglets, resembling the transition from fetal to neonate circulation. However, the cardiac gene expression does not explain plasma concentrations.

Originalsprog	Engelsk
Tidsskrift	Endocrinology
Vol/bind	154
Udgave nummer	5
Sider (fra-til)	1864-1872
Antal sider	9
ISSN	0013-7227
DOI	https://doi.org/10.1210/en.2012-2186
Status	Udgivet - 1 maj 2013

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10.1210/en.2012-2186

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title = "Cardiac natriuretic peptide gene expression and plasma concentrations during the first 72 hours of life in piglets",

abstract = "Plasma measurement of cardiac natriuretic peptides constitutes promising markers of congenital heart disease. However, concentrations change rapidly and dramatically during the first days after delivery even in healthy neonates, which complicates clinical interpretation. It is unknown whether these changes in plasma concentrations are explained by corresponding changes in the cardiac gene expression. We quantified the chamber-specific mRNA levels of ANP (A-type natriuretic peptide) and BNP (B-type natriuretic peptide) and plasma pro-ANP and BNP-32 concentrations in healthy piglets during the first 72 hours of life (from 2 litters, n = 44). Chamber-specific ANP and BNP mRNA levels reflected hemodynamic neonate changes at birth but did not correlate with circulating natriuretic peptide concentrations. However, plasma pro-ANP and creatinine concentrations were closely correlated (P < .0001; r = 0.73). Plasma pro-ANP levels were highest on the day of delivery (5580 pmol/L [4320-6786] decreasing to 2484 pmol/L [1602-2898] after 72 hours, P < .0001). During the 72 hours, gel chromatography suggested that the translational products in circulation and in atrial tissue were immature, ie, unprocessed pro-ANP. In contrast to pro-ANP, BNP-32 plasma concentrations were low at delivery and peaked after 48 hours (12 [10.5-20.6] vs. 88.8 [71.7-101.4] pmol/L, P < .0001). To conclude, ANP and BNP gene expression differs considerably between cardiac chambers in the first 72 hours of life in healthy piglets, resembling the transition from fetal to neonate circulation. However, the cardiac gene expression does not explain plasma concentrations.",

keywords = "Animals, Animals, Newborn, Atrial Natriuretic Factor, Coronary Circulation, Echocardiography, Female, Fetal Blood, Gene Expression, Male, Myocardium, Natriuretic Peptide, Brain, Osmolar Concentration, Pregnancy, Swine, Time Factors",

author = "Julie Smith and Christina Christoffersen and N{\o}rgaard, {Linn Maiken} and Olsen, {Lisbeth H{\o}ier} and Vejlstrup, {Niels G.} and Andersen, {Claus B{\o}gelund} and G{\o}tze, {Jens P.}",

year = "2013",

month = may,

day = "1",

doi = "10.1210/en.2012-2186",

language = "English",

volume = "154",

pages = "1864--1872",

journal = "Journal of Clinical Endocrinology and Metabolism",

issn = "0013-7227",

publisher = "Oxford University Press",

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TY - JOUR

T1 - Cardiac natriuretic peptide gene expression and plasma concentrations during the first 72 hours of life in piglets

AU - Smith, Julie

AU - Christoffersen, Christina

AU - Nørgaard, Linn Maiken

AU - Olsen, Lisbeth Høier

AU - Vejlstrup, Niels G.

AU - Andersen, Claus Bøgelund

AU - Gøtze, Jens P.

PY - 2013/5/1

Y1 - 2013/5/1

N2 - Plasma measurement of cardiac natriuretic peptides constitutes promising markers of congenital heart disease. However, concentrations change rapidly and dramatically during the first days after delivery even in healthy neonates, which complicates clinical interpretation. It is unknown whether these changes in plasma concentrations are explained by corresponding changes in the cardiac gene expression. We quantified the chamber-specific mRNA levels of ANP (A-type natriuretic peptide) and BNP (B-type natriuretic peptide) and plasma pro-ANP and BNP-32 concentrations in healthy piglets during the first 72 hours of life (from 2 litters, n = 44). Chamber-specific ANP and BNP mRNA levels reflected hemodynamic neonate changes at birth but did not correlate with circulating natriuretic peptide concentrations. However, plasma pro-ANP and creatinine concentrations were closely correlated (P < .0001; r = 0.73). Plasma pro-ANP levels were highest on the day of delivery (5580 pmol/L [4320-6786] decreasing to 2484 pmol/L [1602-2898] after 72 hours, P < .0001). During the 72 hours, gel chromatography suggested that the translational products in circulation and in atrial tissue were immature, ie, unprocessed pro-ANP. In contrast to pro-ANP, BNP-32 plasma concentrations were low at delivery and peaked after 48 hours (12 [10.5-20.6] vs. 88.8 [71.7-101.4] pmol/L, P < .0001). To conclude, ANP and BNP gene expression differs considerably between cardiac chambers in the first 72 hours of life in healthy piglets, resembling the transition from fetal to neonate circulation. However, the cardiac gene expression does not explain plasma concentrations.

AB - Plasma measurement of cardiac natriuretic peptides constitutes promising markers of congenital heart disease. However, concentrations change rapidly and dramatically during the first days after delivery even in healthy neonates, which complicates clinical interpretation. It is unknown whether these changes in plasma concentrations are explained by corresponding changes in the cardiac gene expression. We quantified the chamber-specific mRNA levels of ANP (A-type natriuretic peptide) and BNP (B-type natriuretic peptide) and plasma pro-ANP and BNP-32 concentrations in healthy piglets during the first 72 hours of life (from 2 litters, n = 44). Chamber-specific ANP and BNP mRNA levels reflected hemodynamic neonate changes at birth but did not correlate with circulating natriuretic peptide concentrations. However, plasma pro-ANP and creatinine concentrations were closely correlated (P < .0001; r = 0.73). Plasma pro-ANP levels were highest on the day of delivery (5580 pmol/L [4320-6786] decreasing to 2484 pmol/L [1602-2898] after 72 hours, P < .0001). During the 72 hours, gel chromatography suggested that the translational products in circulation and in atrial tissue were immature, ie, unprocessed pro-ANP. In contrast to pro-ANP, BNP-32 plasma concentrations were low at delivery and peaked after 48 hours (12 [10.5-20.6] vs. 88.8 [71.7-101.4] pmol/L, P < .0001). To conclude, ANP and BNP gene expression differs considerably between cardiac chambers in the first 72 hours of life in healthy piglets, resembling the transition from fetal to neonate circulation. However, the cardiac gene expression does not explain plasma concentrations.

KW - Animals

KW - Animals, Newborn

KW - Atrial Natriuretic Factor

KW - Coronary Circulation

KW - Echocardiography

KW - Female

KW - Fetal Blood

KW - Gene Expression

KW - Male

KW - Myocardium

KW - Natriuretic Peptide, Brain

KW - Osmolar Concentration

KW - Pregnancy

KW - Swine

KW - Time Factors

U2 - 10.1210/en.2012-2186

DO - 10.1210/en.2012-2186

M3 - Journal article

C2 - 23539511

SN - 0013-7227

VL - 154

SP - 1864

EP - 1872

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

IS - 5

ER -

Cardiac natriuretic peptide gene expression and plasma concentrations during the first 72 hours of life in piglets

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