Carboxypeptidase-B-like processing of the C-terminus of glucagon-like peptide-2 in pig and human small intestine

TY - JOUR

T1 - Carboxypeptidase-B-like processing of the C-terminus of glucagon-like peptide-2 in pig and human small intestine

AU - Orskov, C

AU - Buhl, T

AU - Rabenhøj, L

AU - Kofod, Hans

AU - Holst, J J

PY - 1989/4/24

Y1 - 1989/4/24

N2 - We developed specific, C-terminal radioimmunoassays for three proglucagon (PG) fragments: PG 151-158, PG 151-160 and PG 126-159 (glucagon-like peptide-2 (GLP-2] in order to determine the exact C-terminal sequence of the newly isolated GLP-2 in man and pig. The antigens and the antisera showed no mutual cross-reactivity. By gel filtration of extracts of pig and human small intestine, the immunoreactivity eluting at the position of GLP-2 was identified by the radioimmunoassays for glucagon-like peptide-2 (PG 126-159) and for PG 151-158, whereas the assay for PG 151-160 was completely negative. We conclude that the C-terminal amino acid residue of pig and human ileal GLP-2 is PG 158. Thus the basic residues, PG 159 and 160 are removed during its processing in the small intestine.

AB - We developed specific, C-terminal radioimmunoassays for three proglucagon (PG) fragments: PG 151-158, PG 151-160 and PG 126-159 (glucagon-like peptide-2 (GLP-2] in order to determine the exact C-terminal sequence of the newly isolated GLP-2 in man and pig. The antigens and the antisera showed no mutual cross-reactivity. By gel filtration of extracts of pig and human small intestine, the immunoreactivity eluting at the position of GLP-2 was identified by the radioimmunoassays for glucagon-like peptide-2 (PG 126-159) and for PG 151-158, whereas the assay for PG 151-160 was completely negative. We conclude that the C-terminal amino acid residue of pig and human ileal GLP-2 is PG 158. Thus the basic residues, PG 159 and 160 are removed during its processing in the small intestine.

KW - Amino Acid Sequence

KW - Animals

KW - Carboxypeptidase B

KW - Carboxypeptidases

KW - Chromatography, Gel

KW - Gastrointestinal Hormones

KW - Glucagon-Like Peptide 2

KW - Glucagon-Like Peptides

KW - Humans

KW - Intestine, Small

KW - Molecular Sequence Data

KW - Peptide Fragments

KW - Peptides

KW - Protein Precursors

KW - Protein Processing, Post-Translational

KW - Radioimmunoassay

KW - Swine

M3 - Journal article

C2 - 2714431

SN - 0014-5793

VL - 247

SP - 193

EP - 196

JO - F E B S Letters

JF - F E B S Letters

IS - 2

ER -