Carbonic anhydrases in human keratinocytes and their regulation by all-trans retinoic acid and 1 alpha,25-dihydroxyvitamin D-3

Bani Kaur Suri; Artur Schmidtchen; Navin Kumar Verma

doi:10.1111/exd.13976

Carbonic anhydrases in human keratinocytes and their regulation by all-trans retinoic acid and 1 alpha,25-dihydroxyvitamin D-3

Bani Kaur Suri, Artur Schmidtchen, Navin Kumar Verma

Biomedicinsk Institut

2 Citationer (Scopus)

Abstract

Carbonic anhydrases (CAs) are ubiquitously expressed enzymes and catalyse an important physiological reaction of interconverting the hydration of carbon dioxide to bicarbonates, which is crucial for maintaining acid/base equilibrium in certain tissues. While 15 different isoforms of CAs are present in various cell types in human tissues, their expression pattern in the epidermis remains to be investigated. Here, we report the expression of 5 CA isoforms (CAII, CAIX, CAXI, CAXII and CAXIII) in human primary keratinocytes. Further, we demonstrate that the expression of CAII and CAIX in these cells is significantly up-regulated by the biologically active metabolites of vitamin A (all-trans retinoic acid) and vitamin D (1 alpha,25-dihydroxyvitamin D-3), respectively. Taken together, apart from providing new information on the expression of CAs in the skin, our results highlight a previously undisclosed connection between vitamin A and CAII expression and vitamin D and CAIX expression.

Originalsprog	Engelsk
Tidsskrift	Experimental Dermatology
Vol/bind	28
Udgave nummer	8
Sider (fra-til)	976-980
ISSN	0906-6705
DOI	https://doi.org/10.1111/exd.13976
Status	Udgivet - aug. 2019

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10.1111/exd.13976

Citationsformater

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title = "Carbonic anhydrases in human keratinocytes and their regulation by all-trans retinoic acid and 1 alpha,25-dihydroxyvitamin D-3",

abstract = "Carbonic anhydrases (CAs) are ubiquitously expressed enzymes and catalyse an important physiological reaction of interconverting the hydration of carbon dioxide to bicarbonates, which is crucial for maintaining acid/base equilibrium in certain tissues. While 15 different isoforms of CAs are present in various cell types in human tissues, their expression pattern in the epidermis remains to be investigated. Here, we report the expression of 5 CA isoforms (CAII, CAIX, CAXI, CAXII and CAXIII) in human primary keratinocytes. Further, we demonstrate that the expression of CAII and CAIX in these cells is significantly up-regulated by the biologically active metabolites of vitamin A (all-trans retinoic acid) and vitamin D (1 alpha,25-dihydroxyvitamin D-3), respectively. Taken together, apart from providing new information on the expression of CAs in the skin, our results highlight a previously undisclosed connection between vitamin A and CAII expression and vitamin D and CAIX expression.",

keywords = "carbonic anhydrases, retinoic acid, vitamin A, vitamin D",

author = "Suri, {Bani Kaur} and Artur Schmidtchen and Verma, {Navin Kumar}",

year = "2019",

month = aug,

doi = "10.1111/exd.13976",

language = "English",

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TY - JOUR

T1 - Carbonic anhydrases in human keratinocytes and their regulation by all-trans retinoic acid and 1 alpha,25-dihydroxyvitamin D-3

AU - Suri, Bani Kaur

AU - Schmidtchen, Artur

AU - Verma, Navin Kumar

PY - 2019/8

Y1 - 2019/8

N2 - Carbonic anhydrases (CAs) are ubiquitously expressed enzymes and catalyse an important physiological reaction of interconverting the hydration of carbon dioxide to bicarbonates, which is crucial for maintaining acid/base equilibrium in certain tissues. While 15 different isoforms of CAs are present in various cell types in human tissues, their expression pattern in the epidermis remains to be investigated. Here, we report the expression of 5 CA isoforms (CAII, CAIX, CAXI, CAXII and CAXIII) in human primary keratinocytes. Further, we demonstrate that the expression of CAII and CAIX in these cells is significantly up-regulated by the biologically active metabolites of vitamin A (all-trans retinoic acid) and vitamin D (1 alpha,25-dihydroxyvitamin D-3), respectively. Taken together, apart from providing new information on the expression of CAs in the skin, our results highlight a previously undisclosed connection between vitamin A and CAII expression and vitamin D and CAIX expression.

AB - Carbonic anhydrases (CAs) are ubiquitously expressed enzymes and catalyse an important physiological reaction of interconverting the hydration of carbon dioxide to bicarbonates, which is crucial for maintaining acid/base equilibrium in certain tissues. While 15 different isoforms of CAs are present in various cell types in human tissues, their expression pattern in the epidermis remains to be investigated. Here, we report the expression of 5 CA isoforms (CAII, CAIX, CAXI, CAXII and CAXIII) in human primary keratinocytes. Further, we demonstrate that the expression of CAII and CAIX in these cells is significantly up-regulated by the biologically active metabolites of vitamin A (all-trans retinoic acid) and vitamin D (1 alpha,25-dihydroxyvitamin D-3), respectively. Taken together, apart from providing new information on the expression of CAs in the skin, our results highlight a previously undisclosed connection between vitamin A and CAII expression and vitamin D and CAIX expression.

KW - carbonic anhydrases

KW - retinoic acid

KW - vitamin A

KW - vitamin D

U2 - 10.1111/exd.13976

DO - 10.1111/exd.13976

M3 - Journal article

C2 - 31125456

SN - 0906-6705

VL - 28

SP - 976

EP - 980

JO - Experimental Dermatology

JF - Experimental Dermatology

IS - 8

ER -

Carbonic anhydrases in human keratinocytes and their regulation by all-trans retinoic acid and 1 alpha,25-dihydroxyvitamin D-3

Abstract

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