TY - JOUR
T1 - Carbon input belowground is the major C flux contributing to leaf litter mass loss
T2 - Evidences from a 13C labelled-leaf litter experiment
AU - Rubino, Mauro
AU - Dungait, null
AU - Evershed, null
AU - Bertolini, null
AU - De Angelis, null
AU - D'Onofrio, null
AU - Lagomarsino, null
AU - Lubritto, null
AU - Merola, null
AU - Terrasi, null
AU - Cotrufo, null
PY - 2010/7/1
Y1 - 2010/7/1
N2 - Partitioning of the quantities of C lost by leaf litter through decomposition into (i) CO2 efflux to the atmosphere and (ii) C input to soil organic matter (SOM) is essential in order to develop a deeper understanding of the litter-soil biogeochemical continuum. However, this is a challenging task due to the occurrence of many different processes contributing to litter biomass loss. With the aim of quantifying different fluxes of C lost by leaf litter decomposition, a field experiment was performed at a short rotation coppice poplar plantation in central Italy. Populus nigra leaf litter, enriched in 13C (δ13C ∼ +160‰) was placed within collars to decompose in direct contact with the soil (δ13C ∼ -26‰) for 11 months. CO2 efflux from within the collars and its isotopic composition were determined at monthly intervals. After 11 months, remaining litter and soil profiles (0-20 cm) were sampled and analysed for their total C and 13C content. Gas chromatography (GC), GC-mass spectrometry (MS) and GC-combustion-isotope ratio (GC/C/IRMS) were used to analyse phospholipid fatty acids (PLFA) extracted from soil samples to identify the groups of soil micro-organisms that had incorporated litter-derived C and to determine the quantity of C incorporated by the soil microbial biomass (SMB). By the end of the experiment, the litter had lost about 80% of its original weight. The fraction of litter C lost as an input into the soil (67 ± 12% of the total C loss) was found to be twice as much as the fraction released as CO2 to the atmosphere (30 ± 3%), thus demonstrating the importance of quantifying litter-derived C input to soils, in litter decomposition studies. The mean δ13C values of PLFAs in soil (δ13C = -12.5‰) showed sustained incorporation of litter-derived C after one year (7.8 ± 1.6% of total PLFA-C). Thus, through the application of stable 13C isotope analyses, we have quantified two major C fluxes contributing to litter decomposition, at macroscopic and microscopic levels.
AB - Partitioning of the quantities of C lost by leaf litter through decomposition into (i) CO2 efflux to the atmosphere and (ii) C input to soil organic matter (SOM) is essential in order to develop a deeper understanding of the litter-soil biogeochemical continuum. However, this is a challenging task due to the occurrence of many different processes contributing to litter biomass loss. With the aim of quantifying different fluxes of C lost by leaf litter decomposition, a field experiment was performed at a short rotation coppice poplar plantation in central Italy. Populus nigra leaf litter, enriched in 13C (δ13C ∼ +160‰) was placed within collars to decompose in direct contact with the soil (δ13C ∼ -26‰) for 11 months. CO2 efflux from within the collars and its isotopic composition were determined at monthly intervals. After 11 months, remaining litter and soil profiles (0-20 cm) were sampled and analysed for their total C and 13C content. Gas chromatography (GC), GC-mass spectrometry (MS) and GC-combustion-isotope ratio (GC/C/IRMS) were used to analyse phospholipid fatty acids (PLFA) extracted from soil samples to identify the groups of soil micro-organisms that had incorporated litter-derived C and to determine the quantity of C incorporated by the soil microbial biomass (SMB). By the end of the experiment, the litter had lost about 80% of its original weight. The fraction of litter C lost as an input into the soil (67 ± 12% of the total C loss) was found to be twice as much as the fraction released as CO2 to the atmosphere (30 ± 3%), thus demonstrating the importance of quantifying litter-derived C input to soils, in litter decomposition studies. The mean δ13C values of PLFAs in soil (δ13C = -12.5‰) showed sustained incorporation of litter-derived C after one year (7.8 ± 1.6% of total PLFA-C). Thus, through the application of stable 13C isotope analyses, we have quantified two major C fluxes contributing to litter decomposition, at macroscopic and microscopic levels.
U2 - 10.1016/j.soilbio.2010.02.018
DO - 10.1016/j.soilbio.2010.02.018
M3 - Journal article
SN - 0038-0717
VL - 42
SP - 1009
EP - 1016
JO - Soil Biology & Biochemistry
JF - Soil Biology & Biochemistry
IS - 7
ER -