Carbohydrate microarray technology applied to high-throughput mapping of plant cell wall glycans using comprehensive microarray polymer profiling (CoMPP)

Stjepan Kresimir Kracun, Jonatan Ulrik Fangel, Maja Gro Rydahl, Henriette Lodberg Pedersen, Silvia Vidal Melgosa, William George Tycho Willats

19 Citationer (Scopus)

Abstract

Cell walls are an important feature of plant cells and a major component of the plant glycome. They have both structural and physiological functions and are critical for plant growth and development. The diversity and complexity of these structures demand advanced high-throughput techniques to answer questions about their structure, functions and roles in both fundamental and applied scientific fields. Microarray technology provides both the high-throughput and the feasibility aspects required to meet that demand. In this chapter, some of the most recent microarray-based techniques relating to plant cell walls are described together with an overview of related contemporary techniques applied to carbohydrate microarrays and their general potential in glycoscience. A detailed experimental procedure for high-throughput mapping of plant cell wall glycans using the comprehensive microarray polymer profiling (CoMPP) technique is included in the chapter and provides a good example of both the robust and high-throughput nature of microarrays as well as their applicability to plant glycomics.

OriginalsprogEngelsk
TitelHigh-throughput glycomics and glycoproteomics
RedaktørerGordan Lauc, Manfred Wuhrer
Antal sider19
ForlagSpringer
Publikationsdato2017
Sider147-165
Kapitel12
ISBN (Trykt)978-1-4939-6491-8
ISBN (Elektronisk)978-1-4939-6493-2
DOI
StatusUdgivet - 2017
NavnMethods in Molecular Biology
Nummer1
Vol/bind1503
ISSN1064-3745

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