@article{69cc634067dd11df928f000ea68e967b,
title = "Calcium-independent phospholipase A2 regulates retinal pigment epithelium proliferation and may be important in the pathogenesis of retinal diseases",
abstract = "Calcium-independent phospholipase A2, group VIA (iPLA2-VIA) is involved in cell proliferation. This study aimed to evaluate the role of iPLA2-VIA in retinal pigment epithelium (RPE) cell proliferation and in retinal diseases involving RPE proliferation. A human RPE cell line (ARPE-19) was used to explore this role in vitro. Proliferating ARPE-19 cells had increased expression and activity of iPLA2-VIA. iPLA2-VIA was found in the nuclei of proliferating ARPE-19 cells, whereas in confluent ARPE-19 cells, with limited proliferation, iPLA2-VIA was primarily found in the cytosol. Inhibition of iPLA2-VIA decreased the rate of proliferation, whereas over expression of iPLA2-VIA increased the rate of proliferation. Using an experimental porcine model of RPE proliferation we demonstrated significant nuclear upregulation of iPLA2-VIA in proliferating RPE cells in vivo. We furthermore evaluated the expression of iPLA2-VIA in proliferative vitreoretinopathy (PVR). PVR membranes revealed nuclear expression of iPLA2-VIA in the RPE cells which had migrated and participated in the formation of the membranes. Overall, the present results point to an important role of iPLA2-VIA in the regulation of RPE proliferation suggesting that iPLA2-VIA may be considered as a possible pharmaceutical target in retinal diseases involving RPE proliferation and migration.",
author = "M Kolko and Kiilgaard, {J F} and J Wang and Poulsen, {K A} and Andreasen, {J R} and {la Cour}, M and Nissen, {M H} and S Heegaard and Bazan, {N G} and Prause, {J U} and M Kolko and Kiilgaard, {J F} and J Wang and Poulsen, {K A} and Andreasen, {J R} and {la Cour}, M and Nissen, {M H} and S Heegaard and Bazan, {N G} and Prause, {J U}",
note = "Keywords: Alternative Splicing; Animals; Cell Nucleus; Cell Proliferation; Cells, Cultured; Endoplasmic Reticulum; Gene Silencing; Humans; Phospholipases A2, Calcium-Independent; RNA, Small Interfering; Retinal Pigment Epithelium; Sus scrofa; Vitreoretinopathy, Proliferative",
year = "2009",
month = sep,
day = "1",
doi = "10.1016/j.exer.2009.04.002",
language = "English",
volume = "89",
pages = "383--91",
journal = "Experimental Eye Research",
issn = "0014-4835",
publisher = "Academic Press",
number = "3",
}
