Bulk culture levels of specific cytotoxic T-cell activity against HIV-1 proteins are not associated with risk of death

TY - JOUR

T1 - Bulk culture levels of specific cytotoxic T-cell activity against HIV-1 proteins are not associated with risk of death

AU - Aladdin, H

AU - Ullum, H

AU - Lepri, A Cozzi

AU - Leffers, H

AU - Katzenstein, T

AU - Gerstoft, J

AU - Gjedde, S B

AU - Phillips, A N

AU - Skinhøj, P

AU - Pedersen, Bente Klarlund

PY - 1999/8

Y1 - 1999/8

N2 - The ability of cytotoxic T lymphocytes (CTL) to control and influence the outcome of human immunodeficiency virus (HIV) infection is not fully understood. The association between HIV-CTL activity and disease progression was evaluated prospectively in 36 HIV-1-infected individuals with a median follow-up of 3.0 years. HIV-CTL activity was measured in a 4 h Cr* release assay using autologous target cells expressing HIV-1 BRU isolate gene products (gp-120, gag, pol, nef) and a bulk culture of autologous effector cells. The CD4 count was measured at enrolment and plasma HIV RNA was measured retrospectively. The present study failed to support the hypothesis that HIV-CTL activity, as measured using the present method, is important in reducing the risk of death in HIV-infected individuals. However, using other approaches and methods could possibly yield other conclusions, and further prospective studies are needed to examine the relationship between CTL and disease progression.

AB - The ability of cytotoxic T lymphocytes (CTL) to control and influence the outcome of human immunodeficiency virus (HIV) infection is not fully understood. The association between HIV-CTL activity and disease progression was evaluated prospectively in 36 HIV-1-infected individuals with a median follow-up of 3.0 years. HIV-CTL activity was measured in a 4 h Cr* release assay using autologous target cells expressing HIV-1 BRU isolate gene products (gp-120, gag, pol, nef) and a bulk culture of autologous effector cells. The CD4 count was measured at enrolment and plasma HIV RNA was measured retrospectively. The present study failed to support the hypothesis that HIV-CTL activity, as measured using the present method, is important in reducing the risk of death in HIV-infected individuals. However, using other approaches and methods could possibly yield other conclusions, and further prospective studies are needed to examine the relationship between CTL and disease progression.

KW - Adult

KW - CD4 Lymphocyte Count

KW - Cells, Cultured

KW - Disease Progression

KW - Female

KW - Follow-Up Studies

KW - Gene Products, gag

KW - Gene Products, nef

KW - Gene Products, pol

KW - HIV Antigens

KW - HIV Envelope Protein gp120

KW - HIV Infections

KW - HIV-1

KW - Humans

KW - Male

KW - Middle Aged

KW - RNA, Viral

KW - Retrospective Studies

KW - Risk Factors

KW - T-Lymphocytes, Cytotoxic

KW - nef Gene Products, Human Immunodeficiency Virus

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Journal article

C2 - 10447929

SN - 0301-6323

VL - 50

SP - 223

EP - 227

JO - Scandinavian Journal of Immunology, Supplement

JF - Scandinavian Journal of Immunology, Supplement

IS - 2

ER -