Bovine mastitis bacteria resolved by MALDI-TOF mass spectrometry

Bettina Nonnemann; Ulrike Lyhs; Line Svennesen; Katja Ann Kristensen; Ilka C. Klaas; Karl Pedersen

doi:10.3168/jds.2018-15424

Bovine mastitis bacteria resolved by MALDI-TOF mass spectrometry

Bettina Nonnemann^*, Ulrike Lyhs, Line Svennesen, Katja Ann Kristensen, Ilka C. Klaas, Karl Pedersen

^*Corresponding author af dette arbejde

20 Citationer (Scopus)

Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a fast and reliable method to identify the most common pathogenic bacteria in humans and animals. The goals of this study were to amend a commercial database with additional species, evaluate the amended database for identification of bacterial genera and species causing bovine mastitis, and describe the plethora of species involved. In total, 500 udder pathogenic isolates were subjected to MALDI-TOF MS using bacterial or fungal colony material; 93.5% could be identified to the species level, and 6.5% were identified only to the genus level. Isolates identified to the genus level required further identification to the species level by conventional methods or 16S rDNA sequencing. Mass spectra from verified species were used to expand the MALDI-TOF MS database to improve future identification ability. A total of 24 genera and 61 species were identified in this study. Identified isolates were mainly staphylococci, streptococci, Enterobacteriaceae, and coryneforme bacteria. In conclusion, MALDI-TOF MS is a powerful, rapid, and reliable technique to identify the most common microorganisms causing bovine mastitis, and the database can be continuously expanded and improved with additional species.

Originalsprog	Engelsk
Tidsskrift	Journal of Dairy Science
Vol/bind	102
Udgave nummer	3
Sider (fra-til)	2515-2524
Antal sider	10
ISSN	0022-0302
DOI	https://doi.org/10.3168/jds.2018-15424
Status	Udgivet - 2019

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10.3168/jds.2018-15424

http://www.journalofdairyscience.org/article/S0022030219300438/pdf

Citationsformater

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title = "Bovine mastitis bacteria resolved by MALDI-TOF mass spectrometry",

abstract = "Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a fast and reliable method to identify the most common pathogenic bacteria in humans and animals. The goals of this study were to amend a commercial database with additional species, evaluate the amended database for identification of bacterial genera and species causing bovine mastitis, and describe the plethora of species involved. In total, 500 udder pathogenic isolates were subjected to MALDI-TOF MS using bacterial or fungal colony material; 93.5% could be identified to the species level, and 6.5% were identified only to the genus level. Isolates identified to the genus level required further identification to the species level by conventional methods or 16S rDNA sequencing. Mass spectra from verified species were used to expand the MALDI-TOF MS database to improve future identification ability. A total of 24 genera and 61 species were identified in this study. Identified isolates were mainly staphylococci, streptococci, Enterobacteriaceae, and coryneforme bacteria. In conclusion, MALDI-TOF MS is a powerful, rapid, and reliable technique to identify the most common microorganisms causing bovine mastitis, and the database can be continuously expanded and improved with additional species.",

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author = "Bettina Nonnemann and Ulrike Lyhs and Line Svennesen and Kristensen, {Katja Ann} and Klaas, {Ilka C.} and Karl Pedersen",

year = "2019",

doi = "10.3168/jds.2018-15424",

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T1 - Bovine mastitis bacteria resolved by MALDI-TOF mass spectrometry

AU - Nonnemann, Bettina

AU - Lyhs, Ulrike

AU - Svennesen, Line

AU - Kristensen, Katja Ann

AU - Klaas, Ilka C.

AU - Pedersen, Karl

PY - 2019

Y1 - 2019

N2 - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a fast and reliable method to identify the most common pathogenic bacteria in humans and animals. The goals of this study were to amend a commercial database with additional species, evaluate the amended database for identification of bacterial genera and species causing bovine mastitis, and describe the plethora of species involved. In total, 500 udder pathogenic isolates were subjected to MALDI-TOF MS using bacterial or fungal colony material; 93.5% could be identified to the species level, and 6.5% were identified only to the genus level. Isolates identified to the genus level required further identification to the species level by conventional methods or 16S rDNA sequencing. Mass spectra from verified species were used to expand the MALDI-TOF MS database to improve future identification ability. A total of 24 genera and 61 species were identified in this study. Identified isolates were mainly staphylococci, streptococci, Enterobacteriaceae, and coryneforme bacteria. In conclusion, MALDI-TOF MS is a powerful, rapid, and reliable technique to identify the most common microorganisms causing bovine mastitis, and the database can be continuously expanded and improved with additional species.

AB - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a fast and reliable method to identify the most common pathogenic bacteria in humans and animals. The goals of this study were to amend a commercial database with additional species, evaluate the amended database for identification of bacterial genera and species causing bovine mastitis, and describe the plethora of species involved. In total, 500 udder pathogenic isolates were subjected to MALDI-TOF MS using bacterial or fungal colony material; 93.5% could be identified to the species level, and 6.5% were identified only to the genus level. Isolates identified to the genus level required further identification to the species level by conventional methods or 16S rDNA sequencing. Mass spectra from verified species were used to expand the MALDI-TOF MS database to improve future identification ability. A total of 24 genera and 61 species were identified in this study. Identified isolates were mainly staphylococci, streptococci, Enterobacteriaceae, and coryneforme bacteria. In conclusion, MALDI-TOF MS is a powerful, rapid, and reliable technique to identify the most common microorganisms causing bovine mastitis, and the database can be continuously expanded and improved with additional species.

KW - bovine mastitis

KW - matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry

KW - rapid identification

U2 - 10.3168/jds.2018-15424

DO - 10.3168/jds.2018-15424

M3 - Journal article

C2 - 30639010

AN - SCOPUS:85059667759

SN - 0022-0302

VL - 102

SP - 2515

EP - 2524

JO - Journal of Dairy Science

JF - Journal of Dairy Science

IS - 3

ER -

Bovine mastitis bacteria resolved by MALDI-TOF mass spectrometry

Abstract

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