Bone healing and graft resorption of autograft, anorganic bovine bone and beta-tricalcium phosphate. A histologic and histomorphometric study in the mandibles of minipigs

Simon Storgård Jensen; Nina Broggini; Erik Hjørting-Hansen; Robert Schenk; Daniel Buser

doi:10.1111/j.1600-0501.2005.01257.x

Bone healing and graft resorption of autograft, anorganic bovine bone and beta-tricalcium phosphate. A histologic and histomorphometric study in the mandibles of minipigs

Simon Storgård Jensen, Nina Broggini, Erik Hjørting-Hansen, Robert Schenk, Daniel Buser

Abstract

OBJECTIVE: The purpose was to qualitatively and quantitatively compare the bone formation and graft resorption of two different bone substitutes used in both orthopedic and oral surgery, with autogenous bone as a positive control.

MATERIALS AND METHODS: Three standardized bone defects were prepared in both mandibular angles of 12 adult minipigs. The defects were grafted with either autograft, anorganic bovine bone (ABB), or synthetic beta-tricalcium phosphate (beta-TCP). Sacrifice was performed after 1, 2, 4, and 8 weeks for histologic and histomorphometric analysis.

RESULTS: At 2 weeks, more new bone formation was seen in defects filled with autograft than with ABB (P approximately 0.0005) and beta-TCP (P approximately 0.002). After 4 weeks, there was no significant difference between beta-TCP and the two other materials. Defects grafted with ABB still exhibited less bone formation as compared with autograft (P approximately 0.004). At 8 weeks, more bone formation was observed in defects grafted with autograft (P approximately 0.003) and beta-TCP (P approximately 0.00004) than with ABB. No difference could be demonstrated between beta-TCP and autograft. beta-TCP resorbed almost completely over 8 weeks, whereas ABB remained stable.

CONCLUSION: Both bone substitutes seemed to decelerate bone regeneration in the early healing phase as compared with autograft. All defects ultimately regenerated with newly formed bone and a developing bone marrow. The grafting materials showed complete osseous integration. Both bone substitutes may have a place in reconstructive surgery where different clinical indications require differences in biodegradability.

Originalsprog	Engelsk
Tidsskrift	Clinical Oral Implants Research
Vol/bind	17
Udgave nummer	3
Sider (fra-til)	237-43
Antal sider	7
ISSN	0905-7161
DOI	https://doi.org/10.1111/j.1600-0501.2005.01257.x
Status	Udgivet - jun. 2006

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10.1111/j.1600-0501.2005.01257.x

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Bone healing and graft resorption of autograft, anorganic bovine bone and beta-tricalcium phosphate. A histologic and histomorphometric study in the mandibles of minipigs. / Jensen, Simon Storgård; Broggini, Nina; Hjørting-Hansen, Erik et al.
I: Clinical Oral Implants Research, Bind 17, Nr. 3, 06.2006, s. 237-43.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

@article{7a891050b5774c64a980f3f4d14b786e,

title = "Bone healing and graft resorption of autograft, anorganic bovine bone and beta-tricalcium phosphate. A histologic and histomorphometric study in the mandibles of minipigs",

abstract = "OBJECTIVE: The purpose was to qualitatively and quantitatively compare the bone formation and graft resorption of two different bone substitutes used in both orthopedic and oral surgery, with autogenous bone as a positive control.MATERIALS AND METHODS: Three standardized bone defects were prepared in both mandibular angles of 12 adult minipigs. The defects were grafted with either autograft, anorganic bovine bone (ABB), or synthetic beta-tricalcium phosphate (beta-TCP). Sacrifice was performed after 1, 2, 4, and 8 weeks for histologic and histomorphometric analysis.RESULTS: At 2 weeks, more new bone formation was seen in defects filled with autograft than with ABB (P approximately 0.0005) and beta-TCP (P approximately 0.002). After 4 weeks, there was no significant difference between beta-TCP and the two other materials. Defects grafted with ABB still exhibited less bone formation as compared with autograft (P approximately 0.004). At 8 weeks, more bone formation was observed in defects grafted with autograft (P approximately 0.003) and beta-TCP (P approximately 0.00004) than with ABB. No difference could be demonstrated between beta-TCP and autograft. beta-TCP resorbed almost completely over 8 weeks, whereas ABB remained stable.CONCLUSION: Both bone substitutes seemed to decelerate bone regeneration in the early healing phase as compared with autograft. All defects ultimately regenerated with newly formed bone and a developing bone marrow. The grafting materials showed complete osseous integration. Both bone substitutes may have a place in reconstructive surgery where different clinical indications require differences in biodegradability.",

keywords = "Animals, Biocompatible Materials/therapeutic use, Bone Marrow/pathology, Bone Regeneration/physiology, Bone Resorption/pathology, Bone Substitutes/therapeutic use, Bone Transplantation/pathology, Calcium Phosphates/therapeutic use, Cattle, Mandible/pathology, Mandibular Diseases/pathology, Osseointegration/physiology, Osteogenesis/physiology, Swine, Swine, Miniature, Time Factors, Transplantation, Autologous, Wound Healing/physiology",

author = "Jensen, {Simon Storg{\aa}rd} and Nina Broggini and Erik Hj{\o}rting-Hansen and Robert Schenk and Daniel Buser",

year = "2006",

month = jun,

doi = "10.1111/j.1600-0501.2005.01257.x",

language = "English",

volume = "17",

pages = "237--43",

journal = "Clinical Oral Implants Research",

issn = "0905-7161",

publisher = "Wiley-Blackwell",

number = "3",

}

TY - JOUR

T1 - Bone healing and graft resorption of autograft, anorganic bovine bone and beta-tricalcium phosphate. A histologic and histomorphometric study in the mandibles of minipigs

AU - Jensen, Simon Storgård

AU - Broggini, Nina

AU - Hjørting-Hansen, Erik

AU - Schenk, Robert

AU - Buser, Daniel

PY - 2006/6

Y1 - 2006/6

N2 - OBJECTIVE: The purpose was to qualitatively and quantitatively compare the bone formation and graft resorption of two different bone substitutes used in both orthopedic and oral surgery, with autogenous bone as a positive control.MATERIALS AND METHODS: Three standardized bone defects were prepared in both mandibular angles of 12 adult minipigs. The defects were grafted with either autograft, anorganic bovine bone (ABB), or synthetic beta-tricalcium phosphate (beta-TCP). Sacrifice was performed after 1, 2, 4, and 8 weeks for histologic and histomorphometric analysis.RESULTS: At 2 weeks, more new bone formation was seen in defects filled with autograft than with ABB (P approximately 0.0005) and beta-TCP (P approximately 0.002). After 4 weeks, there was no significant difference between beta-TCP and the two other materials. Defects grafted with ABB still exhibited less bone formation as compared with autograft (P approximately 0.004). At 8 weeks, more bone formation was observed in defects grafted with autograft (P approximately 0.003) and beta-TCP (P approximately 0.00004) than with ABB. No difference could be demonstrated between beta-TCP and autograft. beta-TCP resorbed almost completely over 8 weeks, whereas ABB remained stable.CONCLUSION: Both bone substitutes seemed to decelerate bone regeneration in the early healing phase as compared with autograft. All defects ultimately regenerated with newly formed bone and a developing bone marrow. The grafting materials showed complete osseous integration. Both bone substitutes may have a place in reconstructive surgery where different clinical indications require differences in biodegradability.

AB - OBJECTIVE: The purpose was to qualitatively and quantitatively compare the bone formation and graft resorption of two different bone substitutes used in both orthopedic and oral surgery, with autogenous bone as a positive control.MATERIALS AND METHODS: Three standardized bone defects were prepared in both mandibular angles of 12 adult minipigs. The defects were grafted with either autograft, anorganic bovine bone (ABB), or synthetic beta-tricalcium phosphate (beta-TCP). Sacrifice was performed after 1, 2, 4, and 8 weeks for histologic and histomorphometric analysis.RESULTS: At 2 weeks, more new bone formation was seen in defects filled with autograft than with ABB (P approximately 0.0005) and beta-TCP (P approximately 0.002). After 4 weeks, there was no significant difference between beta-TCP and the two other materials. Defects grafted with ABB still exhibited less bone formation as compared with autograft (P approximately 0.004). At 8 weeks, more bone formation was observed in defects grafted with autograft (P approximately 0.003) and beta-TCP (P approximately 0.00004) than with ABB. No difference could be demonstrated between beta-TCP and autograft. beta-TCP resorbed almost completely over 8 weeks, whereas ABB remained stable.CONCLUSION: Both bone substitutes seemed to decelerate bone regeneration in the early healing phase as compared with autograft. All defects ultimately regenerated with newly formed bone and a developing bone marrow. The grafting materials showed complete osseous integration. Both bone substitutes may have a place in reconstructive surgery where different clinical indications require differences in biodegradability.

KW - Animals

KW - Biocompatible Materials/therapeutic use

KW - Bone Marrow/pathology

KW - Bone Regeneration/physiology

KW - Bone Resorption/pathology

KW - Bone Substitutes/therapeutic use

KW - Bone Transplantation/pathology

KW - Calcium Phosphates/therapeutic use

KW - Cattle

KW - Mandible/pathology

KW - Mandibular Diseases/pathology

KW - Osseointegration/physiology

KW - Osteogenesis/physiology

KW - Swine

KW - Swine, Miniature

KW - Time Factors

KW - Transplantation, Autologous

KW - Wound Healing/physiology

U2 - 10.1111/j.1600-0501.2005.01257.x

DO - 10.1111/j.1600-0501.2005.01257.x

M3 - Journal article

C2 - 16672017

SN - 0905-7161

VL - 17

SP - 237

EP - 243

JO - Clinical Oral Implants Research

JF - Clinical Oral Implants Research

IS - 3

ER -

Bone healing and graft resorption of autograft, anorganic bovine bone and beta-tricalcium phosphate. A histologic and histomorphometric study in the mandibles of minipigs

Abstract

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