Body fluid identification of blood, saliva and semen using second generation sequencing of micro-RNA

TY - JOUR

T1 - Body fluid identification of blood, saliva and semen using second generation sequencing of micro-RNA

AU - Petersen, Christel H.

AU - Hjort, Benjamin Benn

AU - Tvedebrink, Torben

AU - Kielpinski, Lukasz Jan

AU - Vinther, Jeppe

AU - Morling, Niels

PY - 2013

Y1 - 2013

N2 - We report a new second generation sequencing method for identification micro-RNA (miRNA) that can be used to identify body fluids and tissues. Principal component analysis of 10 miRNAs with high expression in 16 samples of blood, saliva and semen showed clear differences in the expression of miRNA in the investigated tissues. We expect that the method can also be used for identification of other miRNAs that can be used for identifying other body fluids and tissues. We also expect that the method can be used for identification of body fluids and tissues in practical forensic genetic case work.

AB - We report a new second generation sequencing method for identification micro-RNA (miRNA) that can be used to identify body fluids and tissues. Principal component analysis of 10 miRNAs with high expression in 16 samples of blood, saliva and semen showed clear differences in the expression of miRNA in the investigated tissues. We expect that the method can also be used for identification of other miRNAs that can be used for identifying other body fluids and tissues. We also expect that the method can be used for identification of body fluids and tissues in practical forensic genetic case work.

KW - Body fluids

KW - Forensic genetics

KW - Microrna

KW - Second generation sequencing

KW - Tissue identification

U2 - 10.1016/j.fsigss.2013.10.105

DO - 10.1016/j.fsigss.2013.10.105

M3 - Journal article

AN - SCOPUS:84889888082

SN - 1875-1768

VL - 4

SP - e204-5

JO - Forensic Science International: Genetics. Supplement Series

JF - Forensic Science International: Genetics. Supplement Series

IS - 1

ER -