BioFET-SIM web interface: implementation and two applications

TY - JOUR

T1 - BioFET-SIM web interface

T2 - implementation and two applications

AU - Hediger, Martin Robert

AU - Jensen, Jan Halborg

AU - De Vico, Luca

N1 - e45379

PY - 2012/10/8

Y1 - 2012/10/8

N2 - We present a web interface which allows us to conveniently set up calculations based on the BioFET-SIM model. With the interface, the signal of a BioFET sensor can be calculated depending on its parameters, as well as the signal dependence on pH. As an illustration, two case studies are presented. In the first case, a generic peptide with opposite charges on both ends is inverted in orientation on a semiconducting nanowire surface leading to a corresponding change in sign of the computed sensitivity of the device. In the second case, the binding of an antibody/antigen complex on the nanowire surface is studied in terms of orientation and analyte/nanowire surface distance. We demonstrate how the BioFET-SIM web interface can aid in the understanding of experimental data and postulate alternative ways of antibody/antigen orientation on the nanowire surface.

AB - We present a web interface which allows us to conveniently set up calculations based on the BioFET-SIM model. With the interface, the signal of a BioFET sensor can be calculated depending on its parameters, as well as the signal dependence on pH. As an illustration, two case studies are presented. In the first case, a generic peptide with opposite charges on both ends is inverted in orientation on a semiconducting nanowire surface leading to a corresponding change in sign of the computed sensitivity of the device. In the second case, the binding of an antibody/antigen complex on the nanowire surface is studied in terms of orientation and analyte/nanowire surface distance. We demonstrate how the BioFET-SIM web interface can aid in the understanding of experimental data and postulate alternative ways of antibody/antigen orientation on the nanowire surface.

U2 - 10.1371/journal.pone.0045379

DO - 10.1371/journal.pone.0045379

M3 - Journal article

C2 - 23056201

SN - 1932-6203

VL - 7

JO - P L o S One

JF - P L o S One

IS - 10

ER -