BioFET-SIM web interface: implementation and two applications

Martin Robert Hediger; Jan Halborg Jensen; Luca De Vico

doi:10.1371/journal.pone.0045379

BioFET-SIM web interface: implementation and two applications

Martin Robert Hediger, Jan Halborg Jensen, Luca De Vico

Kemisk Institut

6 Citationer (Scopus)

1481 Downloads (Pure)

Abstract

We present a web interface which allows us to conveniently set up calculations based on the BioFET-SIM model. With the interface, the signal of a BioFET sensor can be calculated depending on its parameters, as well as the signal dependence on pH. As an illustration, two case studies are presented. In the first case, a generic peptide with opposite charges on both ends is inverted in orientation on a semiconducting nanowire surface leading to a corresponding change in sign of the computed sensitivity of the device. In the second case, the binding of an antibody/antigen complex on the nanowire surface is studied in terms of orientation and analyte/nanowire surface distance. We demonstrate how the BioFET-SIM web interface can aid in the understanding of experimental data and postulate alternative ways of antibody/antigen orientation on the nanowire surface.

Originalsprog	Engelsk
Tidsskrift	P L o S One
Vol/bind	7
Udgave nummer	10
Antal sider	10
ISSN	1932-6203
DOI	https://doi.org/10.1371/journal.pone.0045379
Status	Udgivet - 8 okt. 2012

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10.1371/journal.pone.0045379

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BioFET-SIM web interface: implementation and two applications

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