Benzyl alcohol induces a reversible fragmentation of the Golgi apparatus and inhibits membrane trafficking between endosomes and the trans-Golgi network

TY - JOUR

T1 - Benzyl alcohol induces a reversible fragmentation of the Golgi apparatus and inhibits membrane trafficking between endosomes and the trans-Golgi network

AU - Simm, Roger

AU - Kvalvaag, Audun Sverre

AU - van Deurs, Bo

AU - Lindbäck, Toril

AU - Sandvig, Kirsten

N1 - Copyright © 2017 Elsevier Inc. All rights reserved.

PY - 2017/8/1

Y1 - 2017/8/1

N2 - Benzyl alcohol (BnOH) is widely used as a component of foods, cosmetics, household products and medical products. It is generally considered to be safe for human use, however, it has been connected to a number of adverse effects, including hypersensitivity reactions and neonatal deaths. BnOH is a membrane fluidizing agent that can affect membrane protein activity and cellular processes such as ligand binding to cell surface receptors, endocytosis and degradation of lysosomal cargo. In this study, we examined the effects of BnOH on intracellular transport using Shiga toxin (Stx), diphtheria toxin (DT) and ricin. BnOH caused reduced toxicity of all three toxins at BnOH concentrations that cause membrane fluidization. The reduced toxicity of Stx and ricin was mainly due to inhibition of retrograde transport between endosomes and the trans-Golgi network as BnOH had small effects on cell association and endocytosis of ricin and Stx. Strikingly, BnOH also induced a reversible fragmentation of the Golgi apparatus.

AB - Benzyl alcohol (BnOH) is widely used as a component of foods, cosmetics, household products and medical products. It is generally considered to be safe for human use, however, it has been connected to a number of adverse effects, including hypersensitivity reactions and neonatal deaths. BnOH is a membrane fluidizing agent that can affect membrane protein activity and cellular processes such as ligand binding to cell surface receptors, endocytosis and degradation of lysosomal cargo. In this study, we examined the effects of BnOH on intracellular transport using Shiga toxin (Stx), diphtheria toxin (DT) and ricin. BnOH caused reduced toxicity of all three toxins at BnOH concentrations that cause membrane fluidization. The reduced toxicity of Stx and ricin was mainly due to inhibition of retrograde transport between endosomes and the trans-Golgi network as BnOH had small effects on cell association and endocytosis of ricin and Stx. Strikingly, BnOH also induced a reversible fragmentation of the Golgi apparatus.

KW - Benzyl Alcohol

KW - Biological Transport

KW - Cell Movement

KW - Endocytosis

KW - Endosomes

KW - Golgi Apparatus

KW - HeLa Cells

KW - Humans

KW - Protein Transport

KW - Shiga Toxin

KW - trans-Golgi Network

KW - Journal Article

U2 - 10.1016/j.yexcr.2017.04.025

DO - 10.1016/j.yexcr.2017.04.025

M3 - Journal article

C2 - 28450044

SN - 0014-4827

VL - 357

SP - 67

EP - 78

JO - Experimental Cell Research

JF - Experimental Cell Research

IS - 1

ER -