Bacterial composition in whole saliva from patients with severe hyposalivation: a case-control study

TY - JOUR

T1 - Bacterial composition in whole saliva from patients with severe hyposalivation

T2 - a case-control study

AU - Belstrøm, Daniel

AU - Holmstrup, Palle

AU - Fiehn, Nils-Erik

AU - Rosing, Kasper

AU - Jensen, Allan Bardow

AU - Paster, Bruce J

AU - Lynge Pedersen, Anne Marie

N1 - This article is protected by copyright. All rights reserved.

PY - 2016/5/1

Y1 - 2016/5/1

N2 - Objective: The purpose of this study was to compare the microbiota of stimulated whole saliva samples from patients with severe hyposalivation to samples from individuals with normal whole saliva flow rates. It was hypothesized that the two groups differ with regard to salivary bacterial profiles. Methods: This cross-sectional study included 36 participants (24 females and 12 males, mean age 58.5 years) with severe hyposalivation and 36 gender-, age-, and geographically matched participants with normal salivary secretion from the Danish Health Examination Survey (DANHES). The microbiota of stimulated whole saliva samples was characterized by HOMINGS. Results: The two groups had comparable caries experience measured by decayed, missed, filled surfaces/teeth and decayed, missed, filled root surfaces as well as active caries lesions. In addition, no single probe target was present with a significant difference in frequency or proportional presence between groups. Furthermore, data reduction by principal component analysis and correspondence analysis showed comparable bacterial community profiles between groups. Conclusions: The results indicate that the salivary bacterial profiles of patients with severe hyposalivation do not differ from those of individuals with normal salivary secretion, when there are virtually no untreated active caries lesions present in the oral cavity.

AB - Objective: The purpose of this study was to compare the microbiota of stimulated whole saliva samples from patients with severe hyposalivation to samples from individuals with normal whole saliva flow rates. It was hypothesized that the two groups differ with regard to salivary bacterial profiles. Methods: This cross-sectional study included 36 participants (24 females and 12 males, mean age 58.5 years) with severe hyposalivation and 36 gender-, age-, and geographically matched participants with normal salivary secretion from the Danish Health Examination Survey (DANHES). The microbiota of stimulated whole saliva samples was characterized by HOMINGS. Results: The two groups had comparable caries experience measured by decayed, missed, filled surfaces/teeth and decayed, missed, filled root surfaces as well as active caries lesions. In addition, no single probe target was present with a significant difference in frequency or proportional presence between groups. Furthermore, data reduction by principal component analysis and correspondence analysis showed comparable bacterial community profiles between groups. Conclusions: The results indicate that the salivary bacterial profiles of patients with severe hyposalivation do not differ from those of individuals with normal salivary secretion, when there are virtually no untreated active caries lesions present in the oral cavity.

U2 - 10.1111/odi.12452

DO - 10.1111/odi.12452

M3 - Journal article

C2 - 26824889

SN - 1354-523X

VL - 22

SP - 330

EP - 337

JO - Oral Diseases

JF - Oral Diseases

IS - 4

ER -