Bacteria as transporters of phosphorus through soil

Nadia Glæsner; Jacob Bælum; Carsten Suhr Jacobsen; Christian Ritz; Gitte Holton Rubæk; Charlotte Kjærgaard; Jakob Magid

doi:10.1111/ejss.12314

Bacteria as transporters of phosphorus through soil

Nadia Glæsner, Jacob Bælum, Carsten Suhr Jacobsen, Christian Ritz, Gitte Holton Rubæk, Charlotte Kjærgaard, Jakob Magid

4 Citationer (Scopus)

Abstract

The transport of phosphorus (P) from agricultural land has led to the eutrophication of surface waters worldwide, especially in areas with intensive animal production. In this research, we investigated the role of bacteria in the leaching of P through three agricultural soils with different textures, before and after the surface application of dairy slurry manure (Bos taurus L.). Leachates of intact soil columns (diameter 20 cm, height 20 cm) of loamy sand, sandy loam and loam were analysed for total bacterial cells measured by universal 16S rRNA genes, and compared with the P content of leachates. The number of 16S rRNA genes per bacterial cell was validated by real-time polymerase chain reaction (PCR) with a universal 16S rRNA gene primer set. Enumeration of bacterial cells by DAPI staining of cells extracted by density gradient centrifugation of the sandy loam estimated 4.6 16S rRNA genes cell^-1. Leaching of bacteria was in the range of 2.5-4.5 × 10⁵ cells ml^-1 prior to application of slurry to the three soil textures. After slurry application, leaching increased to 1.1 × 10⁶ cells ml^-1 in the loamy sand, 4.9 × 10⁶ cells ml^-1 in the sandy loam and 5.0 × 10⁶ cells ml^-1 in the loam. Based on the reported P content of soil bacteria, 0.3-1.8% of the total P leached was present in the bacterial biomass when no slurry was applied, whereas slurry application increased the leaching of P from the bacterial biomass to 3-7.9% of total P leached. Bacterial leaching was related to the particulate P fraction of leachates of the loamy sand and sandy loam, but was also related to both dissolved and particulate leachate fractions of the loam. These results indicate that bacterial transport might play a role in the leaching of P from soil where manure has been applied, but this mechanism of transport is generally not addressed when considering the leaching of P.

Originalsprog	Engelsk
Tidsskrift	European Journal of Soil Science
Vol/bind	67
Udgave nummer	1
Sider (fra-til)	99–108
Antal sider	10
ISSN	1351-0754
DOI	https://doi.org/10.1111/ejss.12314
Status	Udgivet - 1 jan. 2016

Adgang til dokumentet

10.1111/ejss.12314

Citationsformater

@article{777384f66890413e9d60c66a092d1da6,

title = "Bacteria as transporters of phosphorus through soil",

abstract = "The transport of phosphorus (P) from agricultural land has led to the eutrophication of surface waters worldwide, especially in areas with intensive animal production. In this research, we investigated the role of bacteria in the leaching of P through three agricultural soils with different textures, before and after the surface application of dairy slurry manure (Bos taurus L.). Leachates of intact soil columns (diameter 20 cm, height 20 cm) of loamy sand, sandy loam and loam were analysed for total bacterial cells measured by universal 16S rRNA genes, and compared with the P content of leachates. The number of 16S rRNA genes per bacterial cell was validated by real-time polymerase chain reaction (PCR) with a universal 16S rRNA gene primer set. Enumeration of bacterial cells by DAPI staining of cells extracted by density gradient centrifugation of the sandy loam estimated 4.6 16S rRNA genes cell-1. Leaching of bacteria was in the range of 2.5-4.5 × 105 cells ml-1 prior to application of slurry to the three soil textures. After slurry application, leaching increased to 1.1 × 106 cells ml-1 in the loamy sand, 4.9 × 106 cells ml-1 in the sandy loam and 5.0 × 106 cells ml-1 in the loam. Based on the reported P content of soil bacteria, 0.3-1.8% of the total P leached was present in the bacterial biomass when no slurry was applied, whereas slurry application increased the leaching of P from the bacterial biomass to 3-7.9% of total P leached. Bacterial leaching was related to the particulate P fraction of leachates of the loamy sand and sandy loam, but was also related to both dissolved and particulate leachate fractions of the loam. These results indicate that bacterial transport might play a role in the leaching of P from soil where manure has been applied, but this mechanism of transport is generally not addressed when considering the leaching of P.",

author = "Nadia Gl{\ae}sner and Jacob B{\ae}lum and Jacobsen, {Carsten Suhr} and Christian Ritz and Rub{\ae}k, {Gitte Holton} and Charlotte Kj{\ae}rgaard and Jakob Magid",

note = "Special issue on Including Landmark Papers No. 5.",

year = "2016",

month = jan,

day = "1",

doi = "10.1111/ejss.12314",

language = "English",

volume = "67",

pages = "99–108",

journal = "Journal of Soil Sciences",

issn = "1351-0754",

publisher = "Wiley-Blackwell",

number = "1",

}

TY - JOUR

T1 - Bacteria as transporters of phosphorus through soil

AU - Glæsner, Nadia

AU - Bælum, Jacob

AU - Jacobsen, Carsten Suhr

AU - Ritz, Christian

AU - Rubæk, Gitte Holton

AU - Kjærgaard, Charlotte

AU - Magid, Jakob

N1 - Special issue on Including Landmark Papers No. 5.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - The transport of phosphorus (P) from agricultural land has led to the eutrophication of surface waters worldwide, especially in areas with intensive animal production. In this research, we investigated the role of bacteria in the leaching of P through three agricultural soils with different textures, before and after the surface application of dairy slurry manure (Bos taurus L.). Leachates of intact soil columns (diameter 20 cm, height 20 cm) of loamy sand, sandy loam and loam were analysed for total bacterial cells measured by universal 16S rRNA genes, and compared with the P content of leachates. The number of 16S rRNA genes per bacterial cell was validated by real-time polymerase chain reaction (PCR) with a universal 16S rRNA gene primer set. Enumeration of bacterial cells by DAPI staining of cells extracted by density gradient centrifugation of the sandy loam estimated 4.6 16S rRNA genes cell-1. Leaching of bacteria was in the range of 2.5-4.5 × 105 cells ml-1 prior to application of slurry to the three soil textures. After slurry application, leaching increased to 1.1 × 106 cells ml-1 in the loamy sand, 4.9 × 106 cells ml-1 in the sandy loam and 5.0 × 106 cells ml-1 in the loam. Based on the reported P content of soil bacteria, 0.3-1.8% of the total P leached was present in the bacterial biomass when no slurry was applied, whereas slurry application increased the leaching of P from the bacterial biomass to 3-7.9% of total P leached. Bacterial leaching was related to the particulate P fraction of leachates of the loamy sand and sandy loam, but was also related to both dissolved and particulate leachate fractions of the loam. These results indicate that bacterial transport might play a role in the leaching of P from soil where manure has been applied, but this mechanism of transport is generally not addressed when considering the leaching of P.

AB - The transport of phosphorus (P) from agricultural land has led to the eutrophication of surface waters worldwide, especially in areas with intensive animal production. In this research, we investigated the role of bacteria in the leaching of P through three agricultural soils with different textures, before and after the surface application of dairy slurry manure (Bos taurus L.). Leachates of intact soil columns (diameter 20 cm, height 20 cm) of loamy sand, sandy loam and loam were analysed for total bacterial cells measured by universal 16S rRNA genes, and compared with the P content of leachates. The number of 16S rRNA genes per bacterial cell was validated by real-time polymerase chain reaction (PCR) with a universal 16S rRNA gene primer set. Enumeration of bacterial cells by DAPI staining of cells extracted by density gradient centrifugation of the sandy loam estimated 4.6 16S rRNA genes cell-1. Leaching of bacteria was in the range of 2.5-4.5 × 105 cells ml-1 prior to application of slurry to the three soil textures. After slurry application, leaching increased to 1.1 × 106 cells ml-1 in the loamy sand, 4.9 × 106 cells ml-1 in the sandy loam and 5.0 × 106 cells ml-1 in the loam. Based on the reported P content of soil bacteria, 0.3-1.8% of the total P leached was present in the bacterial biomass when no slurry was applied, whereas slurry application increased the leaching of P from the bacterial biomass to 3-7.9% of total P leached. Bacterial leaching was related to the particulate P fraction of leachates of the loamy sand and sandy loam, but was also related to both dissolved and particulate leachate fractions of the loam. These results indicate that bacterial transport might play a role in the leaching of P from soil where manure has been applied, but this mechanism of transport is generally not addressed when considering the leaching of P.

U2 - 10.1111/ejss.12314

DO - 10.1111/ejss.12314

M3 - Journal article

SN - 1351-0754

VL - 67

SP - 99

EP - 108

JO - Journal of Soil Sciences

JF - Journal of Soil Sciences

IS - 1

ER -

Bacteria as transporters of phosphorus through soil

Abstract

Adgang til dokumentet

Fingeraftryk

Citationsformater