TY - JOUR
T1 - B cells exposed to enterobacterial components suppress development of experimental colitis
AU - Schmidt, Esben Gjerløff Wedebye
AU - Larsen, Hjalte List
AU - Kristensen, Nanna Ny
AU - Poulsen, Steen Seier
AU - Claesson, Mogens Helweg
AU - Pedersen, Anders Elm
N1 - Copyright © 2011 Crohn's & Colitis Foundation of America, Inc.
PY - 2012/2
Y1 - 2012/2
N2 - Background: B cells positively contribute to immunity by antigen presentation to CD4 + T cells, cytokine production, and differentiation into antibody secreting plasma cells. Accumulating evidence implies that B cells also possess immunoregulatory functions closely linked to their capability of IL-10 secretion. Methods: Colitis development was followed in CD4 +CD25 - T cell transplanted SCID mice co-transferred with B cells exposed to an enterobacterial extract (ebx-B cells). B and T cell cytokine expression was measured by flow cytometry and enzyme-linked immunosorbent assay (ELISA). Results: We demonstrate that splenic B cells exposed to ebx produce large amounts of IL-10 in vitro and express CD1d and CD5 previously known to be associated with regulatory B cells. In SCID mice transplanted with colitogenic CD4 +CD25 - T cells, co-transfer of ebx-B cells significantly suppressed development of colitis. Suppression was dependent on B cell-derived IL-10, as co-transfer of IL-10 knockout ebx-B cells failed to suppress colitis. Ebx-B cell-mediated suppression of colitis was associated with a decrease in interferon gamma (IFN-γ)-producing T H1 cells and increased frequencies of Foxp3-expressing T cells. Conclusions: These data demonstrate that splenic B cells exposed to enterobacterial components acquire immunosuppressive functions by which they can suppress development of experimental T cell-mediated colitis in an IL-10-dependent way.
AB - Background: B cells positively contribute to immunity by antigen presentation to CD4 + T cells, cytokine production, and differentiation into antibody secreting plasma cells. Accumulating evidence implies that B cells also possess immunoregulatory functions closely linked to their capability of IL-10 secretion. Methods: Colitis development was followed in CD4 +CD25 - T cell transplanted SCID mice co-transferred with B cells exposed to an enterobacterial extract (ebx-B cells). B and T cell cytokine expression was measured by flow cytometry and enzyme-linked immunosorbent assay (ELISA). Results: We demonstrate that splenic B cells exposed to ebx produce large amounts of IL-10 in vitro and express CD1d and CD5 previously known to be associated with regulatory B cells. In SCID mice transplanted with colitogenic CD4 +CD25 - T cells, co-transfer of ebx-B cells significantly suppressed development of colitis. Suppression was dependent on B cell-derived IL-10, as co-transfer of IL-10 knockout ebx-B cells failed to suppress colitis. Ebx-B cell-mediated suppression of colitis was associated with a decrease in interferon gamma (IFN-γ)-producing T H1 cells and increased frequencies of Foxp3-expressing T cells. Conclusions: These data demonstrate that splenic B cells exposed to enterobacterial components acquire immunosuppressive functions by which they can suppress development of experimental T cell-mediated colitis in an IL-10-dependent way.
U2 - 10.1002/ibd.21769
DO - 10.1002/ibd.21769
M3 - Journal article
C2 - 21618359
SN - 1078-0998
VL - 18
SP - 284
EP - 293
JO - Inflammatory Bowel Diseases
JF - Inflammatory Bowel Diseases
IS - 2
ER -