Azone® decreases the buccal mucosal permeation of Diazepam in a concentration-dependent manner via a reservoir effect

TY - JOUR

T1 - Azone® decreases the buccal mucosal permeation of Diazepam in a concentration-dependent manner via a reservoir effect

AU - Meng-Lund, Emil

AU - Jacobsen, Jette

AU - Jin, Liang

AU - Janfelt, Christian

AU - Holm, René

AU - Müllertz, Anette

AU - Nicolazzo, Joseph A

N1 - © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association.

PY - 2014/4

Y1 - 2014/4

N2 - The purpose of this study was to examine concentration-dependent effects of Azone® (AZ) on the buccal absorption of diazepam (DIAZ). Porcine buccal mucosa was placed in modified Ussing chambers and pretreated with 10 μL of 0%, 5%, 20%, and 50% (w/v) AZ in ethanol. DIAZ was administered to the donor chamber either in solution or a chitosan-based gel. The donor chamber disappearance, receptor chamber appearance, and tissue retention of DIAZ were monitored over 2 h by HPLC, with AZ tissue disposition also measured by liquid chromatography-mass spectrometry profiling of tissue cryosections. DIAZ steady-state flux values significantly (p

AB - The purpose of this study was to examine concentration-dependent effects of Azone® (AZ) on the buccal absorption of diazepam (DIAZ). Porcine buccal mucosa was placed in modified Ussing chambers and pretreated with 10 μL of 0%, 5%, 20%, and 50% (w/v) AZ in ethanol. DIAZ was administered to the donor chamber either in solution or a chitosan-based gel. The donor chamber disappearance, receptor chamber appearance, and tissue retention of DIAZ were monitored over 2 h by HPLC, with AZ tissue disposition also measured by liquid chromatography-mass spectrometry profiling of tissue cryosections. DIAZ steady-state flux values significantly (p

U2 - 10.1002/jps.23877

DO - 10.1002/jps.23877

M3 - Journal article

C2 - 24532052

SN - 0022-3549

VL - 103

SP - 1133

EP - 1141

JO - Journal of Pharmaceutical Sciences

JF - Journal of Pharmaceutical Sciences

IS - 4

M1 - 103

ER -