TY - JOUR
T1 - Autoantibodies to molecular targets in neutrophils in patients with ulcerative colitis
AU - Brimnes, Jens
AU - Nielsen, Ole H.
AU - Wiik, Allan
AU - Heegaard, Niels H.H.
PY - 1999/1/1
Y1 - 1999/1/1
N2 - Autoantibodies against neutrophil granulocytes are frequently observed in patients with ulcerative colitis, but a precise description of the autoantigens involved is lacking. Therefore, sera from 75 patients with ulcerative colitis were studied for antibody specificities by means of indirect immunofluorescence microscopy, enzyme-linked immunosorbent assays, and immunoblotting of extracts of neutrophils, neutrophil granules and lymphocytes. Fifty-six percent of the sera reacted in indirect immunofluorescence with ethanol-fixed neutrophils. On formalin-fixed cells most sera shifted fluorescence pattern, indicating a cytoplasmic origin of antigen(s). Only a few sera reacted with specific, known antigens. Immunoblots showed reactions with a broad panel of antigens with preferences for proteins around 55-65 kDa, which were present in azurophilic granules and in cytosol, an 80-kDa protein found in the specific granules, and a 110-kDa unknown cytosol component. In conclusion, neutrophil-specific IgG autoantibodies from ulcerative colitis patients react with several different antigens, most of which are of nonnuclear origin.
AB - Autoantibodies against neutrophil granulocytes are frequently observed in patients with ulcerative colitis, but a precise description of the autoantigens involved is lacking. Therefore, sera from 75 patients with ulcerative colitis were studied for antibody specificities by means of indirect immunofluorescence microscopy, enzyme-linked immunosorbent assays, and immunoblotting of extracts of neutrophils, neutrophil granules and lymphocytes. Fifty-six percent of the sera reacted in indirect immunofluorescence with ethanol-fixed neutrophils. On formalin-fixed cells most sera shifted fluorescence pattern, indicating a cytoplasmic origin of antigen(s). Only a few sera reacted with specific, known antigens. Immunoblots showed reactions with a broad panel of antigens with preferences for proteins around 55-65 kDa, which were present in azurophilic granules and in cytosol, an 80-kDa protein found in the specific granules, and a 110-kDa unknown cytosol component. In conclusion, neutrophil-specific IgG autoantibodies from ulcerative colitis patients react with several different antigens, most of which are of nonnuclear origin.
KW - Anti-neutrophil cytoplasmic antibodies
KW - Autoantigens
KW - Neutrophils
KW - Ulcerative colitis
UR - http://www.scopus.com/inward/record.url?scp=0032914819&partnerID=8YFLogxK
U2 - 10.1023/A:1026683224057
DO - 10.1023/A:1026683224057
M3 - Journal article
C2 - 10063932
AN - SCOPUS:0032914819
SN - 0163-2116
VL - 44
SP - 415
EP - 423
JO - Digestive Diseases and Sciences
JF - Digestive Diseases and Sciences
IS - 2
ER -