ATAF1 transcription factor directly regulates abscisic acid biosynthetic gene NCED3 in Arabidopsis thaliana

TY - JOUR

T1 - ATAF1 transcription factor directly regulates abscisic acid biosynthetic gene NCED3 in Arabidopsis thaliana

AU - Jensen, Michael Krogh

AU - Lindemose, Søren

AU - Masi, Federico de

AU - Reimer, Julia J.

AU - Nielsen, Michael

AU - Perera, Venura

AU - Workman, Chris T.

AU - Turck, Franziska

AU - Grant, Murray R.

AU - Mundy, John

AU - Petersen, Morten

AU - Skriver, Karen

PY - 2013

Y1 - 2013

N2 - ATAF1, an Arabidopsis thaliana NAC transcription factor, plays important roles in plant adaptation to environmental stress and development. To search for ATAF1 target genes, we used protein binding microarrays and chromatin-immunoprecipitation (ChIP). This identified T[A,C,G]CGT[A,G] and TT[A,C,G]CGT as ATAF1 consensus binding sequences. Co-expression analysis across publicly available microarray experiments identified 25 genes co-expressed with ATAF1. The promoter regions of ATAF1 co-expressors were significantly enriched for ATAF1 binding sites, and TTGCGTA was identified in the promoter of the key abscisic acid (ABA) phytohormone biosynthetic gene NCED3. ChIP-qPCR and expression analysis showed that ATAF1 binding to the NCED3 promoter correlated with increased NCED3 expression and ABA hormone levels. These results indicate that ATAF1 regulates ABA biosynthesis.

AB - ATAF1, an Arabidopsis thaliana NAC transcription factor, plays important roles in plant adaptation to environmental stress and development. To search for ATAF1 target genes, we used protein binding microarrays and chromatin-immunoprecipitation (ChIP). This identified T[A,C,G]CGT[A,G] and TT[A,C,G]CGT as ATAF1 consensus binding sequences. Co-expression analysis across publicly available microarray experiments identified 25 genes co-expressed with ATAF1. The promoter regions of ATAF1 co-expressors were significantly enriched for ATAF1 binding sites, and TTGCGTA was identified in the promoter of the key abscisic acid (ABA) phytohormone biosynthetic gene NCED3. ChIP-qPCR and expression analysis showed that ATAF1 binding to the NCED3 promoter correlated with increased NCED3 expression and ABA hormone levels. These results indicate that ATAF1 regulates ABA biosynthesis.

KW - Arabidopsis

KW - NAC transcription factor

KW - DNA-binding

KW - Abscisic acid biosynthesis

U2 - 10.1016/j.fob.2013.07.006

DO - 10.1016/j.fob.2013.07.006

M3 - Journal article

SN - 2211-5463

VL - 3

SP - 321

EP - 327

JO - FEBS Open Bio

JF - FEBS Open Bio

ER -