TY - JOUR
T1 - Associations between olfactory pathway gene methylation marks, obesity features and dietary intakes
AU - Ramos-Lopez, Omar
AU - Riezu-Boj, Jose I.
AU - Milagro, Fermin I.
AU - Zulet, M. Angeles
AU - Santos, Jose L.
AU - Martinez, J. Alfredo
AU - Alonso, A.
AU - Arancibia, C.
AU - Arós, F.
AU - Astrup, Arne
AU - Babio, N.
AU - Blázquez, V.
AU - Bondia-Pons, I.
AU - Brennan, L.
AU - Buil-Cosiales, P.
AU - Campión, J.
AU - Cataldo, L. R.
AU - Celis-Morales, C.
AU - Corella, D.
AU - Covas, M. I.
AU - Dalskov, S.
AU - Daniel, H.
AU - De Arce, A.
AU - De La Iglesia, R.
AU - Estruch, R.
AU - Fernández-Crehuet, J.
AU - Fiol, M.
AU - Fitó, M.
AU - Flores, M.
AU - Forga, L.
AU - Galgani, J.
AU - Gibney, E. R.
AU - Gibney, M. J.
AU - Gómez-Úriz, A. M.
AU - González-Muniesa, P.
AU - Goyenechea, E.
AU - Guy-Grand, B.
AU - Handjieva-Darlenska, T.
AU - Holst, C.
AU - Huerta, A. E.
AU - Jebb, S.
AU - Kafatos, A.
AU - Larsen, Thomas Meinert
AU - Pedersen, O.
AU - Petersen, M.
AU - Pfeiffer, A.
AU - Sørensen, Thorkild I. A.
AU - Sorlí, J. V.
AU - Toubro, S.
AU - Verdich, C.
AU - MENA Project
N1 - CURIS 2019 NEXS 169
PY - 2019
Y1 - 2019
N2 - Background: Olfaction is an important sense influencing food preferences, appetite, and eating behaviors. This hypothesis-driven study aimed to assess associations between olfactory pathway gene methylation signatures, obesity features, and dietary intakes. Methods: A nutriepigenomic analysis was conducted in 474 adults from the Methyl Epigenome Network Association (MENA) project. Anthropometric measurements, clinical data, and serum metabolic profiles of the study population were obtained from structured databases of the MENA cohorts. Habitual dietary intake was assessed using a validated semiquantitative food frequency questionnaire. DNA methylation was measured in circulating white blood cells by microarray (Infinium Human Methylation 450 K BeadChips). FDR values (p < 0.0001) were used to select those CpGs that showed the best correlation with body mass index (BMI) and waist circumference (WC). Pathway analyses involving the characterization of genes involved in the olfactory transduction system were performed using KEGG and pathDIP reference databases. Results: Overall, 15 CpG sites at olfactory pathway genes were associated with BMI (p < 0.0001) and WC (p < 0.0001) after adjustments for potential confounding factors. Together, methylation levels at the15 CpG sites accounted for 22% and 20% of the variability in BMI and WC (r 2 = 0.219, p < 0.001, and r 2 = 0.204, p < 0.001, respectively). These genes encompassed olfactory receptors (OR4D2, OR51A7, OR2T34, and OR2Y1) and several downstream signaling molecules (SLC8A1, ANO2, PDE2A, CALML3, GNG7, CALML6, PRKG1, and CAMK2D), which significantly regulated odor detection and signal transduction processes within the complete olfactory cascade, as revealed by pathway enrichment analyses (p = 1.94 × 10 -10 ). Moreover, OR4D2 and OR2Y1 gene methylation patterns strongly correlated with daily intakes of total energy (p < 0.0001), carbohydrates (p < 0.0001), protein (p < 0.0001), and fat (p < 0.0001).Conclusions: The results of this study suggest novel relationships between olfactory pathway gene methylation signatures, obesity indices, and dietary intakes.
AB - Background: Olfaction is an important sense influencing food preferences, appetite, and eating behaviors. This hypothesis-driven study aimed to assess associations between olfactory pathway gene methylation signatures, obesity features, and dietary intakes. Methods: A nutriepigenomic analysis was conducted in 474 adults from the Methyl Epigenome Network Association (MENA) project. Anthropometric measurements, clinical data, and serum metabolic profiles of the study population were obtained from structured databases of the MENA cohorts. Habitual dietary intake was assessed using a validated semiquantitative food frequency questionnaire. DNA methylation was measured in circulating white blood cells by microarray (Infinium Human Methylation 450 K BeadChips). FDR values (p < 0.0001) were used to select those CpGs that showed the best correlation with body mass index (BMI) and waist circumference (WC). Pathway analyses involving the characterization of genes involved in the olfactory transduction system were performed using KEGG and pathDIP reference databases. Results: Overall, 15 CpG sites at olfactory pathway genes were associated with BMI (p < 0.0001) and WC (p < 0.0001) after adjustments for potential confounding factors. Together, methylation levels at the15 CpG sites accounted for 22% and 20% of the variability in BMI and WC (r 2 = 0.219, p < 0.001, and r 2 = 0.204, p < 0.001, respectively). These genes encompassed olfactory receptors (OR4D2, OR51A7, OR2T34, and OR2Y1) and several downstream signaling molecules (SLC8A1, ANO2, PDE2A, CALML3, GNG7, CALML6, PRKG1, and CAMK2D), which significantly regulated odor detection and signal transduction processes within the complete olfactory cascade, as revealed by pathway enrichment analyses (p = 1.94 × 10 -10 ). Moreover, OR4D2 and OR2Y1 gene methylation patterns strongly correlated with daily intakes of total energy (p < 0.0001), carbohydrates (p < 0.0001), protein (p < 0.0001), and fat (p < 0.0001).Conclusions: The results of this study suggest novel relationships between olfactory pathway gene methylation signatures, obesity indices, and dietary intakes.
KW - Diet
KW - Epigenetics
KW - Olfactory system
KW - OR2Y1
KW - OR4D2
KW - Smell
UR - http://www.scopus.com/inward/record.url?scp=85065243457&partnerID=8YFLogxK
U2 - 10.1186/s12263-019-0635-9
DO - 10.1186/s12263-019-0635-9
M3 - Journal article
C2 - 31057674
AN - SCOPUS:85065243457
SN - 1555-8932
VL - 14
JO - Genes & Nutrition
JF - Genes & Nutrition
M1 - 11
ER -
