Association of an Osteopontin gene promoter polymorphism with susceptibility to diabetic nephropathy in Asian Indians

Balneek Singh Cheema; Sreenivasa Iyengar; Tarun Veer Singh Ahluwalia; Harbir Singh Kohli; Rajni Sharma; Viral N Shah; Anil Bhansali; V Sakhuja; Madhu Khullar

doi:10.1016/j.cca.2012.04.028

Association of an Osteopontin gene promoter polymorphism with susceptibility to diabetic nephropathy in Asian Indians

Balneek Singh Cheema, Sreenivasa Iyengar, Tarun Veer Singh Ahluwalia, Harbir Singh Kohli, Rajni Sharma, Viral N Shah, Anil Bhansali, V Sakhuja, Madhu Khullar

4 Citationer (Scopus)

Abstract

Genetic predisposition has been proposed to be a major determinant in the development of renal complications of diabetes. Osteopontin (OPN) has been suggested to be associated with renal diseases characterized by tubulointerstitial fibrosis and proteinuria. However, information on association of genetic polymorphisms in OPN with diabetic nephropathy is lacking. Thus, the present study was designed with the aim to examine the association of an OPN gene promoter polymorphism with diabetic nephropathy in Asian Indians. OPN C-443T (rs11730582) polymorphism was determined in 1115 type 2 diabetic patients belonging to two independently ascertained cohorts using Real time PCR based Taqman assay. We observed a nearly threefold elevated risk of diabetic nephropathy among carriers of T allele and TT genotype of OPN C-443T polymorphism. Further, this allele was found to be significantly associated with proteinuria and lower eGFR, a hallmark of diabetic nephropathy, in both our cohorts. This is the first study which suggests that OPN C-443T polymorphism may be a significant risk factor for diabetic nephropathy in type 2 diabetic patients.

Originalsprog	Engelsk
Tidsskrift	Clinica chimica acta; international journal of clinical chemistry
Vol/bind	413
Udgave nummer	19-20
Sider (fra-til)	1600-4
Antal sider	5
DOI	https://doi.org/10.1016/j.cca.2012.04.028
Status	Udgivet - 9 okt. 2012

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10.1016/j.cca.2012.04.028

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Cheema, B. S., Iyengar, S., Ahluwalia, T. V. S., Kohli, H. S., Sharma, R., Shah, V. N., Bhansali, A., Sakhuja, V., & Khullar, M. (2012). Association of an Osteopontin gene promoter polymorphism with susceptibility to diabetic nephropathy in Asian Indians. Clinica chimica acta; international journal of clinical chemistry, 413(19-20), 1600-4. https://doi.org/10.1016/j.cca.2012.04.028

Association of an Osteopontin gene promoter polymorphism with susceptibility to diabetic nephropathy in Asian Indians. / Cheema, Balneek Singh; Iyengar, Sreenivasa; Ahluwalia, Tarun Veer Singh et al.

I: Clinica chimica acta; international journal of clinical chemistry, Bind 413, Nr. 19-20, 09.10.2012, s. 1600-4.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Cheema, BS, Iyengar, S, Ahluwalia, TVS, Kohli, HS, Sharma, R, Shah, VN, Bhansali, A, Sakhuja, V & Khullar, M 2012, 'Association of an Osteopontin gene promoter polymorphism with susceptibility to diabetic nephropathy in Asian Indians', Clinica chimica acta; international journal of clinical chemistry, bind 413, nr. 19-20, s. 1600-4. https://doi.org/10.1016/j.cca.2012.04.028

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title = "Association of an Osteopontin gene promoter polymorphism with susceptibility to diabetic nephropathy in Asian Indians",

abstract = "Genetic predisposition has been proposed to be a major determinant in the development of renal complications of diabetes. Osteopontin (OPN) has been suggested to be associated with renal diseases characterized by tubulointerstitial fibrosis and proteinuria. However, information on association of genetic polymorphisms in OPN with diabetic nephropathy is lacking. Thus, the present study was designed with the aim to examine the association of an OPN gene promoter polymorphism with diabetic nephropathy in Asian Indians. OPN C-443T (rs11730582) polymorphism was determined in 1115 type 2 diabetic patients belonging to two independently ascertained cohorts using Real time PCR based Taqman assay. We observed a nearly threefold elevated risk of diabetic nephropathy among carriers of T allele and TT genotype of OPN C-443T polymorphism. Further, this allele was found to be significantly associated with proteinuria and lower eGFR, a hallmark of diabetic nephropathy, in both our cohorts. This is the first study which suggests that OPN C-443T polymorphism may be a significant risk factor for diabetic nephropathy in type 2 diabetic patients.",

keywords = "Aged, Alleles, Asian Continental Ancestry Group, Diabetes Mellitus, Type 2, Diabetic Nephropathies, Female, Gene Frequency, Genetic Predisposition to Disease, Glomerular Filtration Rate, Humans, India, Kidney, Male, Middle Aged, Osteopontin, Polymorphism, Single Nucleotide, Promoter Regions, Genetic, Proteinuria, Risk Factors",

author = "Cheema, {Balneek Singh} and Sreenivasa Iyengar and Ahluwalia, {Tarun Veer Singh} and Kohli, {Harbir Singh} and Rajni Sharma and Shah, {Viral N} and Anil Bhansali and V Sakhuja and Madhu Khullar",

year = "2012",

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doi = "10.1016/j.cca.2012.04.028",

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journal = "Clinica Chimica Acta",

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T1 - Association of an Osteopontin gene promoter polymorphism with susceptibility to diabetic nephropathy in Asian Indians

AU - Cheema, Balneek Singh

AU - Iyengar, Sreenivasa

AU - Ahluwalia, Tarun Veer Singh

AU - Kohli, Harbir Singh

AU - Sharma, Rajni

AU - Shah, Viral N

AU - Bhansali, Anil

AU - Sakhuja, V

AU - Khullar, Madhu

PY - 2012/10/9

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N2 - Genetic predisposition has been proposed to be a major determinant in the development of renal complications of diabetes. Osteopontin (OPN) has been suggested to be associated with renal diseases characterized by tubulointerstitial fibrosis and proteinuria. However, information on association of genetic polymorphisms in OPN with diabetic nephropathy is lacking. Thus, the present study was designed with the aim to examine the association of an OPN gene promoter polymorphism with diabetic nephropathy in Asian Indians. OPN C-443T (rs11730582) polymorphism was determined in 1115 type 2 diabetic patients belonging to two independently ascertained cohorts using Real time PCR based Taqman assay. We observed a nearly threefold elevated risk of diabetic nephropathy among carriers of T allele and TT genotype of OPN C-443T polymorphism. Further, this allele was found to be significantly associated with proteinuria and lower eGFR, a hallmark of diabetic nephropathy, in both our cohorts. This is the first study which suggests that OPN C-443T polymorphism may be a significant risk factor for diabetic nephropathy in type 2 diabetic patients.

AB - Genetic predisposition has been proposed to be a major determinant in the development of renal complications of diabetes. Osteopontin (OPN) has been suggested to be associated with renal diseases characterized by tubulointerstitial fibrosis and proteinuria. However, information on association of genetic polymorphisms in OPN with diabetic nephropathy is lacking. Thus, the present study was designed with the aim to examine the association of an OPN gene promoter polymorphism with diabetic nephropathy in Asian Indians. OPN C-443T (rs11730582) polymorphism was determined in 1115 type 2 diabetic patients belonging to two independently ascertained cohorts using Real time PCR based Taqman assay. We observed a nearly threefold elevated risk of diabetic nephropathy among carriers of T allele and TT genotype of OPN C-443T polymorphism. Further, this allele was found to be significantly associated with proteinuria and lower eGFR, a hallmark of diabetic nephropathy, in both our cohorts. This is the first study which suggests that OPN C-443T polymorphism may be a significant risk factor for diabetic nephropathy in type 2 diabetic patients.

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KW - Alleles

KW - Asian Continental Ancestry Group

KW - Diabetes Mellitus, Type 2

KW - Diabetic Nephropathies

KW - Female

KW - Gene Frequency

KW - Genetic Predisposition to Disease

KW - Glomerular Filtration Rate

KW - Humans

KW - India

KW - Kidney

KW - Male

KW - Middle Aged

KW - Osteopontin

KW - Polymorphism, Single Nucleotide

KW - Promoter Regions, Genetic

KW - Proteinuria

KW - Risk Factors

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DO - 10.1016/j.cca.2012.04.028

M3 - Journal article

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SN - 0009-8981

VL - 413

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EP - 1604

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

IS - 19-20

ER -

Association of an Osteopontin gene promoter polymorphism with susceptibility to diabetic nephropathy in Asian Indians

Abstract

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