Assessment of Schistosoma mansoni induced intestinal inflammation by means of eosinophil cationic protein, eosinophil protein X and myeloperoxidase before and after treatment with praziquantel

TY - JOUR

T1 - Assessment of Schistosoma mansoni induced intestinal inflammation by means of eosinophil cationic protein, eosinophil protein X and myeloperoxidase before and after treatment with praziquantel

AU - Reimert, Claus Michael

AU - Tukahebwa, Edridah M.

AU - Kabatereine, Narcis B.

AU - Dunne, David W.

AU - Vennervald, Birgitte J

PY - 2008

Y1 - 2008

N2 - Faecal concentrations of eosinophil cationic protein (ECP), eosinophil protein X (EPX) and myeloperoxidase (MPO) were measured in extracts of stool samplesobtained from a cohort of people (n=182) living in Bugoigo, a fishing communityon the Eastern shore of Lake Albert, Buliisa District, in North Western Ugandawhere Schistosoma mansoni is endemic. Samples were collected before treatment and5, 15, 20 and 52 weeks after treatment with praziquantel. Significantly increasedlevels of faecal ECP and EPX were found in S. mansoni infected individuals(n=155) compared to the levels found in stools from non-infected (n=27) (medianvalues ECP: 11.3 microg/g vs. 5.9 microg/g, P=0.005, and EPX: 413.5 ng/g vs.232.2 ng/g, P=0.045). An increased level of MPO was also found among the infectedindividuals compared to the non-infected 11.6 mu/g vs. 5.3 mu/g, P=0.07).Significant but weak correlations were found between faecal egg counts and faecalconcentrations of ECP and EPX. Treatment with praziquantel induced a significant decline in both ECP and EPX, but only a non-significant reduction in faecal MPO. Following reinfection and despite of very low infection intensities, the protein levels increased significantly reaching the pre-treatment level (ECP and EPX) or levels significantly higher than the pre-treatment levels (MPO). This responsepattern may imply a rebound effect during reinfection following treatment andresolution of immune regulatory immunosuppressive mechanisms in function duringthe chronic infection.

AB - Faecal concentrations of eosinophil cationic protein (ECP), eosinophil protein X (EPX) and myeloperoxidase (MPO) were measured in extracts of stool samplesobtained from a cohort of people (n=182) living in Bugoigo, a fishing communityon the Eastern shore of Lake Albert, Buliisa District, in North Western Ugandawhere Schistosoma mansoni is endemic. Samples were collected before treatment and5, 15, 20 and 52 weeks after treatment with praziquantel. Significantly increasedlevels of faecal ECP and EPX were found in S. mansoni infected individuals(n=155) compared to the levels found in stools from non-infected (n=27) (medianvalues ECP: 11.3 microg/g vs. 5.9 microg/g, P=0.005, and EPX: 413.5 ng/g vs.232.2 ng/g, P=0.045). An increased level of MPO was also found among the infectedindividuals compared to the non-infected 11.6 mu/g vs. 5.3 mu/g, P=0.07).Significant but weak correlations were found between faecal egg counts and faecalconcentrations of ECP and EPX. Treatment with praziquantel induced a significant decline in both ECP and EPX, but only a non-significant reduction in faecal MPO. Following reinfection and despite of very low infection intensities, the protein levels increased significantly reaching the pre-treatment level (ECP and EPX) or levels significantly higher than the pre-treatment levels (MPO). This responsepattern may imply a rebound effect during reinfection following treatment andresolution of immune regulatory immunosuppressive mechanisms in function duringthe chronic infection.

KW - Former LIFE faculty

U2 - 10.1016/j.actatropica.2007.11.004

DO - 10.1016/j.actatropica.2007.11.004

M3 - Journal article

C2 - 18177822

SN - 0001-706X

VL - 105

SP - 253

EP - 259

JO - Acta Tropica

JF - Acta Tropica

IS - 3

ER -