@inbook{0ad588e067fe443292ba0a63ca95ec70,
title = "Assay of flippase activity in proteoliposomes using fluorescent lipid derivatives",
abstract = "Specific membrane proteins, termed lipid flippases, play a central role in facilitating the movement of lipids across cellular membranes. In this protocol, we describe the reconstitution of ATP-driven lipid flippases in liposomes and the analysis of their in vitro flippase activity based on the use of fluorescent lipid derivatives. Working with purified and reconstituted systems provides a well-defined experimental setup and allows to directly characterize these membrane proteins at the molecular level.",
keywords = "ATP-Binding Cassette Transporters, Adenosine Triphosphate, Biological Transport, Cell Membrane, Dithionite, Fluorescence, Lipids, Membrane Proteins, Molecular Biology, Proteolipids, Journal Article, Research Support, Non-U.S. Gov't",
author = "Magdalena Marek and Thomas G{\"u}nther-Pomorski",
year = "2016",
month = jan,
day = "1",
doi = "10.1007/978-1-4939-3179-8_18",
language = "English",
isbn = "978-1-4939-3178-1",
volume = "2.",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "181--191",
editor = "Maike Bublitz",
booktitle = "P-Type ATPases",
}