Arginine-vasopressin stimulates the formation of phosphatidic acid in rat Leydig cells

TY - JOUR

T1 - Arginine-vasopressin stimulates the formation of phosphatidic acid in rat Leydig cells

AU - Nielsen, J.R.

AU - Hansen, Harald S.

AU - Jensen, B.

PY - 1987/1/1

Y1 - 1987/1/1

N2 - Arginine-vasopressin (AVP) stimulated the formation of labelled phosphatidic acid (PA) in [C]arachidonic acid-prelabelled rat Leydig cells. After addition of 10 M AVP [C]arachidonoylphosphatidic acid reached a maximum within 2 min. The increase was dose-dependent (10-10 M). No change in labelling of other phospholipids and diacylglycerol could be detected. The V antagonist dPTyr(Me)AVP inhibited in a dose-dependent manner the AVP-stimulated accumulation of PA. The V agonist dPVDAVP was without effect. The present results suggest that AVP binds to V receptors in rat Leydig cells resulting in stimulation of PA turnover. We suggest that the AVP-stimulated PA formation is an indication of phosphoinositide turnover.

AB - Arginine-vasopressin (AVP) stimulated the formation of labelled phosphatidic acid (PA) in [C]arachidonic acid-prelabelled rat Leydig cells. After addition of 10 M AVP [C]arachidonoylphosphatidic acid reached a maximum within 2 min. The increase was dose-dependent (10-10 M). No change in labelling of other phospholipids and diacylglycerol could be detected. The V antagonist dPTyr(Me)AVP inhibited in a dose-dependent manner the AVP-stimulated accumulation of PA. The V agonist dPVDAVP was without effect. The present results suggest that AVP binds to V receptors in rat Leydig cells resulting in stimulation of PA turnover. We suggest that the AVP-stimulated PA formation is an indication of phosphoinositide turnover.

UR - http://www.scopus.com/inward/record.url?scp=0023179164&partnerID=8YFLogxK

M3 - Journal article

AN - SCOPUS:0023179164

SN - 0014-5793

VL - 218

SP - 93

EP - 96

JO - F E B S Letters

JF - F E B S Letters

IS - 1

ER -