Antisense RNA-regulated programmed cell death

K Gerdes; AP Gultyaev; T Franch; K Pedersen; ND Mikkelsen

doi:10.1146/annurev.genet.31.1.1

Antisense RNA-regulated programmed cell death

K Gerdes, AP Gultyaev, T Franch, K Pedersen, ND Mikkelsen

168 Citationer (Scopus)

Abstract

Eubacterial plasmids and chromosomes encode multiple killer genes belonging to the hok gene family. The plasmid-encoded killer genes mediate plasmid stabilization by killing plasmid-free cells. This review describes the genetics, molecular biology, and evolution of the hok gene family. The complicated antisense RNA-regulated control-loop that regulates posttranscriptional and postsegregational activation of killer mRNA translation in plasmid-free cells is described in detail. Nucleotide covariations in the mRNAs reveal metastable stem-loop structures that are formed at the mRNA 5' ends in the nascent transcripts. The metastable structures prevent translation and antisense RNA binding during transcription. Coupled nucleotide covariations provide evidence for a phylogenetically conserved mRNA folding pathway that involves sequential dynamic RNA rearrangements. Our analyses have elucidated an intricate mechanism by which translation of an antisense RNA-regulated mRNA can be conditionally activated. The complex phylogenetic relationships of the plasmid-and chromosome-encoded systems are also presented and discussed.

Originalsprog	Engelsk
Tidsskrift	Annual Review of Genetics
Vol/bind	31
Sider (fra-til)	1-31
Antal sider	31
ISSN	0066-4197
DOI	https://doi.org/10.1146/annurev.genet.31.1.1
Status	Udgivet - 1997
Udgivet eksternt	Ja

Adgang til dokumentet

10.1146/annurev.genet.31.1.1

Citationsformater

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title = "Antisense RNA-regulated programmed cell death",

abstract = "Eubacterial plasmids and chromosomes encode multiple killer genes belonging to the hok gene family. The plasmid-encoded killer genes mediate plasmid stabilization by killing plasmid-free cells. This review describes the genetics, molecular biology, and evolution of the hok gene family. The complicated antisense RNA-regulated control-loop that regulates posttranscriptional and postsegregational activation of killer mRNA translation in plasmid-free cells is described in detail. Nucleotide covariations in the mRNAs reveal metastable stem-loop structures that are formed at the mRNA 5' ends in the nascent transcripts. The metastable structures prevent translation and antisense RNA binding during transcription. Coupled nucleotide covariations provide evidence for a phylogenetically conserved mRNA folding pathway that involves sequential dynamic RNA rearrangements. Our analyses have elucidated an intricate mechanism by which translation of an antisense RNA-regulated mRNA can be conditionally activated. The complex phylogenetic relationships of the plasmid-and chromosome-encoded systems are also presented and discussed.",

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T1 - Antisense RNA-regulated programmed cell death

AU - Gerdes, K

AU - Gultyaev, AP

AU - Franch, T

AU - Pedersen, K

AU - Mikkelsen, ND

PY - 1997

Y1 - 1997

N2 - Eubacterial plasmids and chromosomes encode multiple killer genes belonging to the hok gene family. The plasmid-encoded killer genes mediate plasmid stabilization by killing plasmid-free cells. This review describes the genetics, molecular biology, and evolution of the hok gene family. The complicated antisense RNA-regulated control-loop that regulates posttranscriptional and postsegregational activation of killer mRNA translation in plasmid-free cells is described in detail. Nucleotide covariations in the mRNAs reveal metastable stem-loop structures that are formed at the mRNA 5' ends in the nascent transcripts. The metastable structures prevent translation and antisense RNA binding during transcription. Coupled nucleotide covariations provide evidence for a phylogenetically conserved mRNA folding pathway that involves sequential dynamic RNA rearrangements. Our analyses have elucidated an intricate mechanism by which translation of an antisense RNA-regulated mRNA can be conditionally activated. The complex phylogenetic relationships of the plasmid-and chromosome-encoded systems are also presented and discussed.

AB - Eubacterial plasmids and chromosomes encode multiple killer genes belonging to the hok gene family. The plasmid-encoded killer genes mediate plasmid stabilization by killing plasmid-free cells. This review describes the genetics, molecular biology, and evolution of the hok gene family. The complicated antisense RNA-regulated control-loop that regulates posttranscriptional and postsegregational activation of killer mRNA translation in plasmid-free cells is described in detail. Nucleotide covariations in the mRNAs reveal metastable stem-loop structures that are formed at the mRNA 5' ends in the nascent transcripts. The metastable structures prevent translation and antisense RNA binding during transcription. Coupled nucleotide covariations provide evidence for a phylogenetically conserved mRNA folding pathway that involves sequential dynamic RNA rearrangements. Our analyses have elucidated an intricate mechanism by which translation of an antisense RNA-regulated mRNA can be conditionally activated. The complex phylogenetic relationships of the plasmid-and chromosome-encoded systems are also presented and discussed.

U2 - 10.1146/annurev.genet.31.1.1

DO - 10.1146/annurev.genet.31.1.1

M3 - Journal article

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SN - 0066-4197

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JO - Annual Review of Genetics

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