Antimicrobial activity and pH of a endodontic sealer containing MTA

TY - ABST

T1 - Antimicrobial activity and pH of a endodontic sealer containing MTA

AU - Maliza, Amanda GA

AU - de Andrade, Flaviana Bombarda

AU - Arias, Marcela C

AU - Chadi, Silvia Fernandes

AU - Benetti, Ana Raquel

AU - Duarte, Marco Antonio Húngaro

AU - Weckwerth, Paulo H

PY - 2012

Y1 - 2012

N2 - Objective: To investigate the antimicrobial activity, calcium release, and pH of a new mineral trioxide aggregate endodontic sealer when compared to endodontic sealers containing calcium hydroxide and/or epoxy resin. Method: Specimens were fabricated from MTA Fillapex, Sealer 26, Sealapex, and AH Plus immediately, 24 or 48 hours prior to the tests. The antimicrobial activity against Enterococcus faecalis and Candida albicans was evaluated by the direct contact and the agar diffusion methods. Calcium release was determined by atomic absorption spectrometry. The pH from solutions containing the sealers was measured using a pHmeter. Results: MTA Fillapex, AH Plus and Sealer 26 resulted in partial inhibition of E. faecalis, while Sealapex resulted in the highest bacterium growth. MTA Fillapex and AH Plus also inhibited to a greater extent the growth of C. albicans. None of the investigated sealers could completely inhibit the growth of Enterococcus faecalis and Candida albicans, but influenced microbial growth to different extent. Significantly higher calcium was released from Sealer 26, followed by Sealapex, MTA Fillapex, and AH Plus in a descending order. All investigated sealers resulted in solution with an alkaline pH. Conclusion: The new mineral trioxide aggregate endodontic sealer presented higher antimicrobial activity when compared to the sealers containing calcium hydroxide and/or epoxy resin. As for pH and calcium release, the sealers containing calcium hydroxide resulted in presented the highest values.

AB - Objective: To investigate the antimicrobial activity, calcium release, and pH of a new mineral trioxide aggregate endodontic sealer when compared to endodontic sealers containing calcium hydroxide and/or epoxy resin. Method: Specimens were fabricated from MTA Fillapex, Sealer 26, Sealapex, and AH Plus immediately, 24 or 48 hours prior to the tests. The antimicrobial activity against Enterococcus faecalis and Candida albicans was evaluated by the direct contact and the agar diffusion methods. Calcium release was determined by atomic absorption spectrometry. The pH from solutions containing the sealers was measured using a pHmeter. Results: MTA Fillapex, AH Plus and Sealer 26 resulted in partial inhibition of E. faecalis, while Sealapex resulted in the highest bacterium growth. MTA Fillapex and AH Plus also inhibited to a greater extent the growth of C. albicans. None of the investigated sealers could completely inhibit the growth of Enterococcus faecalis and Candida albicans, but influenced microbial growth to different extent. Significantly higher calcium was released from Sealer 26, followed by Sealapex, MTA Fillapex, and AH Plus in a descending order. All investigated sealers resulted in solution with an alkaline pH. Conclusion: The new mineral trioxide aggregate endodontic sealer presented higher antimicrobial activity when compared to the sealers containing calcium hydroxide and/or epoxy resin. As for pH and calcium release, the sealers containing calcium hydroxide resulted in presented the highest values.

M3 - Conference abstract for conference

T2 - IADR General Session

Y2 - 20 June 2012 through 23 June 2012

ER -