Angiogenic response to passive movement and active exercise in individuals with peripheral arterial disease

TY - JOUR

T1 - Angiogenic response to passive movement and active exercise in individuals with peripheral arterial disease

AU - Høier, Birgitte

AU - Walker, Meegan

AU - Passos, Madla

AU - Walker, Philip J

AU - Green, Anita

AU - Bangsbo, Jens

AU - Askew, Christopher D

AU - Hellsten, Ylva

N1 - CURIS 2013 NEXS 268

PY - 2013/12/15

Y1 - 2013/12/15

N2 - Angiogenic response to passive movement and active exercise in individuals with peripheral arterial disease. J Appl Physiol 115: 17771787, 2013. First published October 24, 2013; doi:10.1152/japplphysiol.00979.2013.Peripheral arterial disease (PAD) is caused by atherosclerosis and is associated with microcirculatory impairments in skeletal muscle. The present study evaluated the angiogenic response to exercise and passive movement in skeletal muscle of PAD patients compared with healthy control subjects. Twenty-one PAD patients and 17 aged control subjects were randomly assigned to either a passive movement or an active exercise study. Interstitial fluid microdialysate and tissue samples were obtained from the thigh skeletal muscle. Muscle dialysate vascular endothelial growth factor (VEGF) levels were modestly increased in response to either passive movement or active exercise in both subject groups. The basal muscle dialysate level of the angiostatic factor thrombospondin-1 protein was markedly higher (P < 0.05) in PAD patients compared with the control subjects, whereas soluble VEGF receptor-1 dialysate levels were similar in the two groups. The basal VEGF protein content in the muscle tissue samples was 27% lower (P < 0.05) in the PAD patients compared with the control subjects. Analysis of mRNA expression for a range of angiogenic and angiostatic factors revealed a modest change with active exercise and passive movement in both groups, except for an increase (P < 0.05) in the ratio of angiopoietin-2 to angiopoietin-1 mRNA in the PAD group with both interventions. PAD patients and aged individuals showed a similar limited angiogenic response to active exercise and passive movement. The limited increase in muscle extracellular VEGF combined with an elevated basal level of thrombospondin-1 in muscle extracellular fluid of PAD patients may restrict capillary growth in these patients.

AB - Angiogenic response to passive movement and active exercise in individuals with peripheral arterial disease. J Appl Physiol 115: 17771787, 2013. First published October 24, 2013; doi:10.1152/japplphysiol.00979.2013.Peripheral arterial disease (PAD) is caused by atherosclerosis and is associated with microcirculatory impairments in skeletal muscle. The present study evaluated the angiogenic response to exercise and passive movement in skeletal muscle of PAD patients compared with healthy control subjects. Twenty-one PAD patients and 17 aged control subjects were randomly assigned to either a passive movement or an active exercise study. Interstitial fluid microdialysate and tissue samples were obtained from the thigh skeletal muscle. Muscle dialysate vascular endothelial growth factor (VEGF) levels were modestly increased in response to either passive movement or active exercise in both subject groups. The basal muscle dialysate level of the angiostatic factor thrombospondin-1 protein was markedly higher (P < 0.05) in PAD patients compared with the control subjects, whereas soluble VEGF receptor-1 dialysate levels were similar in the two groups. The basal VEGF protein content in the muscle tissue samples was 27% lower (P < 0.05) in the PAD patients compared with the control subjects. Analysis of mRNA expression for a range of angiogenic and angiostatic factors revealed a modest change with active exercise and passive movement in both groups, except for an increase (P < 0.05) in the ratio of angiopoietin-2 to angiopoietin-1 mRNA in the PAD group with both interventions. PAD patients and aged individuals showed a similar limited angiogenic response to active exercise and passive movement. The limited increase in muscle extracellular VEGF combined with an elevated basal level of thrombospondin-1 in muscle extracellular fluid of PAD patients may restrict capillary growth in these patients.

U2 - 10.1152/japplphysiol.00979.2013

DO - 10.1152/japplphysiol.00979.2013

M3 - Journal article

C2 - 24157526

SN - 8750-7587

VL - 115

SP - 1777

EP - 1787

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

IS - 12

ER -