Analyzing temperature-induced transitions in disordered proteins by NMR spectroscopy and secondary chemical shift analyses

Magnus Kjærgaard, Flemming Martin Poulsen, Birthe Brandt Kragelund

Abstract

Intrinsically disordered proteins are abundant in nature and perform many important physiological functions. Multidimensional NMR spectroscopy has been crucial for the understanding of the conformational properties of disordered proteins and is increasingly used to probe their conformational ensembles. Compared to folded proteins, disordered proteins are more malleable and more easily perturbed by environmental factors. Accordingly, the experimental conditions and especially the temperature modify the structural and functional properties of disordered proteins. This chapter discusses practical aspects of NMR studies of temperature-induced structural changes in disordered proteins using chemical shifts.
OriginalsprogEngelsk
TitelIntrinsically disordered protein analysis : methods and experimental tools
RedaktørerVladimir N. Uversky, A. Keith Dunker
Antal sider8
Vol/bind2
ForlagSpringer
Publikationsdato2012
Sider249-256
Kapitel16
ISBN (Trykt)978-1-4614-3703-1
ISBN (Elektronisk)978-1-4614-3704-8
DOI
StatusUdgivet - 2012
NavnMethods in Molecular Biology
Vol/bind896
ISSN1064-3745

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