Analysis of Vδ1 T cells in clinical grade melanoma-infiltrating lymphocytes

TY - JOUR

T1 - Analysis of Vδ1 T cells in clinical grade melanoma-infiltrating lymphocytes

AU - Donia, Marco

AU - Ellebaek, Eva

AU - Andersen, Mads Hald

AU - Straten, Per Thor

AU - Svane, Inge Marie

PY - 2012

Y1 - 2012

N2 - γδ T cells, including Vδ1 and Vδ2 T cells, can recognize tumor-associated ligands neglected by conventional αβ T cells in a MHC -independent manner. Little is known regarding the anticancer potential and the possibility to isolate and expand Vd1 T cells to therapeutically relevant numbers. In this study, we have detected low frequencies of Vδ1 T cells among tumor-infiltrating lymphocyte (TIL) products for adoptive cell transfer generated from melanoma metastases. An increased frequency of Vδ1 T cells was found among the cell products from patients with an advanced disease stage. Vδ1 T cells displayed in vitro antitumor activities and sufficient proliferative potential to generate over 1 × 109 cells using current protocols for T cell transfer. Infusion of Vδ1 T cells together with high numbers of αβ TILs in a clinical trial was safe and well tolerated. These data suggest that Vδ1 T cells should be further scrutinized as a potentially useful tool for the treatment of patients with metastatic melanoma.

AB - γδ T cells, including Vδ1 and Vδ2 T cells, can recognize tumor-associated ligands neglected by conventional αβ T cells in a MHC -independent manner. Little is known regarding the anticancer potential and the possibility to isolate and expand Vd1 T cells to therapeutically relevant numbers. In this study, we have detected low frequencies of Vδ1 T cells among tumor-infiltrating lymphocyte (TIL) products for adoptive cell transfer generated from melanoma metastases. An increased frequency of Vδ1 T cells was found among the cell products from patients with an advanced disease stage. Vδ1 T cells displayed in vitro antitumor activities and sufficient proliferative potential to generate over 1 × 109 cells using current protocols for T cell transfer. Infusion of Vδ1 T cells together with high numbers of αβ TILs in a clinical trial was safe and well tolerated. These data suggest that Vδ1 T cells should be further scrutinized as a potentially useful tool for the treatment of patients with metastatic melanoma.

U2 - 10.4161/onci.21659

DO - 10.4161/onci.21659

M3 - Journal article

C2 - 23243593

SN - 2162-4011

VL - 1

SP - 1297

EP - 1304

JO - OncoImmunology

JF - OncoImmunology

IS - 8

ER -