Analysis of epigenetic modifications of DNA in human cells

TY - JOUR

T1 - Analysis of epigenetic modifications of DNA in human cells

AU - Kristensen, Lasse Sommer

AU - Treppendahl, Marianne Bach

AU - Grønbæk, Kirsten

PY - 2013

Y1 - 2013

N2 - Epigenetics, the study of somatically heritable changes in gene expression not related to changes in the DNA sequence, is a rapidly expanding research field that plays important roles in healthy as well as in diseased cells. DNA methylation and hydroxymethylation are epigenetic modifications found in human cells, which are deeply implicated in normal cellular processes aswell as in several major human diseases. Here, a range of different methods for the analyses of DNA methylation and hydroxymethylation at locus-specific and genome-wide scales is described.

AB - Epigenetics, the study of somatically heritable changes in gene expression not related to changes in the DNA sequence, is a rapidly expanding research field that plays important roles in healthy as well as in diseased cells. DNA methylation and hydroxymethylation are epigenetic modifications found in human cells, which are deeply implicated in normal cellular processes aswell as in several major human diseases. Here, a range of different methods for the analyses of DNA methylation and hydroxymethylation at locus-specific and genome-wide scales is described.

U2 - 10.1002/0471142905.hg2002s77

DO - 10.1002/0471142905.hg2002s77

M3 - Journal article

C2 - 23595599

SN - 1934-8266

VL - Suppl. 77

JO - Current Protocols in Human Genetics

JF - Current Protocols in Human Genetics

M1 - Unit20.2

ER -