TY - JOUR
T1 - Analysis of collagen preservation in bones recovered in archaeological contexts using NIR Hyperspectral Imaging
AU - Vincke, Damien
AU - Miller, Rebecca
AU - Stassart, Édith
AU - Otte, Marcel
AU - Dardenne, Pierre
AU - Collins, Matthew
AU - Wilkinson, Keith
AU - Stewart, John
AU - Baeten, Vincent
AU - Fernández Pierna, Juan Antonio
PY - 2014
Y1 - 2014
N2 - The scope of this article is to propose an innovative method based on Near Infrared Hyperspectral Chemical Imaging (NIR-HCI) to rapidly and non-destructively evaluate the relative degree of collagen preservation in bones recovered from archaeological contexts. This preliminary study has allowed the evaluation of the potential of the method using bone samples from the Early Upper Palaeolithic, Mesolithic and Neolithic periods at the site of Trou AlWesse in Belgium. NIR-HCI, combined with chemometric tools, has identified specific spectral bands characteristic of collagen. A chemometric model has been built using Partial Least Squares Discriminant Analysis (PLS-DA) to identify bones with and without collagen. This enables the evaluation of the degree of collagen preservation and homogeneity in bones within and between different strata, which has direct implications for archaeological applications (e.g., taphonomic analyses, assemblage integrity) and sample selection for subsequent analyses requiring collagen. Two archaeological applications are presented: comparison between sub-layers in an Early Upper Palaeolithic unit, and evaluation of the range of variability in collagen preservation within a single Holocene stratum.
AB - The scope of this article is to propose an innovative method based on Near Infrared Hyperspectral Chemical Imaging (NIR-HCI) to rapidly and non-destructively evaluate the relative degree of collagen preservation in bones recovered from archaeological contexts. This preliminary study has allowed the evaluation of the potential of the method using bone samples from the Early Upper Palaeolithic, Mesolithic and Neolithic periods at the site of Trou AlWesse in Belgium. NIR-HCI, combined with chemometric tools, has identified specific spectral bands characteristic of collagen. A chemometric model has been built using Partial Least Squares Discriminant Analysis (PLS-DA) to identify bones with and without collagen. This enables the evaluation of the degree of collagen preservation and homogeneity in bones within and between different strata, which has direct implications for archaeological applications (e.g., taphonomic analyses, assemblage integrity) and sample selection for subsequent analyses requiring collagen. Two archaeological applications are presented: comparison between sub-layers in an Early Upper Palaeolithic unit, and evaluation of the range of variability in collagen preservation within a single Holocene stratum.
KW - Bone
KW - Collagen
KW - Near Infrared (NIR) Hyperspectral Imaging
KW - Partial Least Squares-Discriminant Analysis (PLS-DA)
KW - Principal Component Analysis (PCA)
KW - Sorting
U2 - 10.1016/j.talanta.2014.02.044
DO - 10.1016/j.talanta.2014.02.044
M3 - Journal article
C2 - 24840431
AN - SCOPUS:84897100860
SN - 0039-9140
VL - 125
SP - 181
EP - 188
JO - Talanta
JF - Talanta
ER -