An updated and simplified method for bile duct cannulation of rats

TY - JOUR

T1 - An updated and simplified method for bile duct cannulation of rats

AU - Tønsberg, Henrik

AU - Holm, René

AU - Bjerregaaard, T. G.

AU - Jacobsen, Jette

AU - Müllertz, Anette

PY - 2010/10

Y1 - 2010/10

N2 - The single bile duct cannulated rat model was used for short-term intestinal absorption studies. Rats were randomly assigned to three groups: (A) bile duct cannulated, (B) control laparotomy and (C) no treatment. The body weight and health of the rats were monitored before and until day 5 after surgery, while bile flow was measured in group A on day 2. On the fifth day, tail vein blood was harvested, and alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and total bilirubin were quantified. Analysis of variance showed no significant difference between the groups for these parameters. This study demonstrated the suitability of a surgical rat model feasible for evaluation of the impact of bile in pharmacokinetic studies.

AB - The single bile duct cannulated rat model was used for short-term intestinal absorption studies. Rats were randomly assigned to three groups: (A) bile duct cannulated, (B) control laparotomy and (C) no treatment. The body weight and health of the rats were monitored before and until day 5 after surgery, while bile flow was measured in group A on day 2. On the fifth day, tail vein blood was harvested, and alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and total bilirubin were quantified. Analysis of variance showed no significant difference between the groups for these parameters. This study demonstrated the suitability of a surgical rat model feasible for evaluation of the impact of bile in pharmacokinetic studies.

KW - Former Faculty of Pharmaceutical Sciences

U2 - 10.1258/la.2010.01001

DO - 10.1258/la.2010.01001

M3 - Journal article

SN - 0023-6772

VL - 44

SP - 373

EP - 376

JO - Laboratory Animals

JF - Laboratory Animals

IS - 4

ER -