An improved method for the immunological detection of mineral bound protein using hydrofluoric acid and direct capture

TY - JOUR

T1 - An improved method for the immunological detection of mineral bound protein using hydrofluoric acid and direct capture

AU - Craig, Oliver E.

AU - Collins, Matthew J.

PY - 2000/3/6

Y1 - 2000/3/6

N2 - Immunological detection of proteins adsorbed to mineral and ceramic surfaces has proved not only difficult but controversial. Unlike the immunological detection of proteins associated with carbonate or phosphate minerals (e.g. shells and bones) proteins adsorbed to siliceous minerals cannot readily be removed by dissolution of the mineral phase. We have previously examined alternative extraction methodologies which claim to bring the protein into solution, but found none of these to be effective. Here we report a novel strategy for immuno-detection of proteins adsorbed to siliceous minerals, the Digestion and Capture Immunoassay (DACIA). The method involves the use of cold, concentrated (4M) hydrofluoric acid (HF) with the simultaneous capture of liberated protein onto a solid phase. The combination of low temperatures and surface stabilisation enables us to detect epitopes from even partially degraded proteins. The method may have a wide application in forensic, archaeological, soil and earth sciences.

AB - Immunological detection of proteins adsorbed to mineral and ceramic surfaces has proved not only difficult but controversial. Unlike the immunological detection of proteins associated with carbonate or phosphate minerals (e.g. shells and bones) proteins adsorbed to siliceous minerals cannot readily be removed by dissolution of the mineral phase. We have previously examined alternative extraction methodologies which claim to bring the protein into solution, but found none of these to be effective. Here we report a novel strategy for immuno-detection of proteins adsorbed to siliceous minerals, the Digestion and Capture Immunoassay (DACIA). The method involves the use of cold, concentrated (4M) hydrofluoric acid (HF) with the simultaneous capture of liberated protein onto a solid phase. The combination of low temperatures and surface stabilisation enables us to detect epitopes from even partially degraded proteins. The method may have a wide application in forensic, archaeological, soil and earth sciences.

KW - DACIA

KW - ELISA

KW - Hydrofluoric acid

KW - Mineral surface

KW - Monoclonal antibodies

KW - Protein extraction

UR - http://www.scopus.com/inward/record.url?scp=0033975964&partnerID=8YFLogxK

U2 - 10.1016/S0022-1759(99)00242-2

DO - 10.1016/S0022-1759(99)00242-2

M3 - Journal article

C2 - 10699582

AN - SCOPUS:0033975964

SN - 0022-1759

VL - 236

SP - 89

EP - 97

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1-2

ER -