@article{f6cd17c051a611dd8d9f000ea68e967b,
title = "An alternative pathway for gene regulation by Myc.",
abstract = "The c-Myc protein activates transcription as part of a heteromeric complex with Max. However, Myc-transformed cells are characterized by loss of expression of several genes, suggesting that Myc may also repress gene expression. Two-hybrid cloning identifies a novel POZ domain Zn finger protein (Miz-1; Myc-interacting Zn finger protein-1) that specifically interacts with Myc, but not with Max or USF. Miz-1 binds to start sites of the adenovirus major late and cyclin D1 promoters and activates transcription from both promoters. Miz-1 has a potent growth arrest function. Binding of Myc to Miz-1 requires the helix-loop-helix domain of Myc and a short amphipathic helix located in the carboxy-terminus of Miz-1. Expression of Myc inhibits transactivation, overcomes Miz-1-induced growth arrest and renders Miz-1 insoluble in vivo. These processes depend on Myc and Miz-1 association and on the integrity of the POZ domain of Miz-1, suggesting that Myc binding activates a latent inhibitory function of this domain. Fusion of a nuclear localization signal induces efficient nuclear transport of Miz-1 and impairs the ability of Myc to overcome transcriptional activation and growth arrest by Miz-1. Our data suggest a model for how gene repression by Myc may occur in vivo.",
author = "K Peukert and P Staller and A Schneider and G Carmichael and F H{\"a}nel and M Eilers",
note = "Keywords: 3T3 Cells; Adenoviridae; Amino Acid Sequence; Animals; Base Sequence; Cyclin D1; DNA-Binding Proteins; Gene Expression Regulation; Gene Library; Genes, Reporter; Hela Cells; Helix-Loop-Helix Motifs; Humans; Kruppel-Like Transcription Factors; Mice; Molecular Sequence Data; Polymerase Chain Reaction; Promoter Regions (Genetics); Protein Biosynthesis; Proto-Oncogene Proteins c-myc; Recombinant Fusion Proteins; Transcription Factors; Transcription, Genetic; Transfection; Viral Proteins; Zinc Fingers",
year = "1997",
doi = "10.1093/emboj/16.18.5672",
language = "English",
volume = "16",
pages = "5672--86",
journal = "E M B O Journal",
issn = "0261-4189",
publisher = "Wiley-Blackwell",
number = "18",
}