Amyloid beta-peptide(25-35) changes [Ca2+] in hippocampal neurons

TY - JOUR

T1 - Amyloid beta-peptide(25-35) changes [Ca2+] in hippocampal neurons

AU - Mogensen, Helle Smidt

AU - Beatty, D M

AU - Morris, S J

AU - Jorgensen, O S

N1 - Keywords: Amyloid beta-Protein; Animals; Animals, Newborn; Astrocytes; Benzopyrans; Calcium; Cell Survival; Cells, Cultured; Fluorescent Dyes; Glial Fibrillary Acidic Protein; Hippocampus; Hydrogen-Ion Concentration; Indoles; Kinetics; Microscopy, Fluorescence; Naphthols; Neurons; Peptide Fragments; Rats; Rhodamines; Time Factors

PY - 1998

Y1 - 1998

N2 - Insoluble aggregates of the amyloid beta-peptide (A beta) is a major constituent of senile plaques found in brains of Alzheimer disease (AD) patients. The detrimental effects of aggregated A beta is associated with an increased intracellular Ca2+ concentration ([Ca2+]i). We examined the effects of A beta(25-35) on [Ca2+]i and intracellular H+ concentration ([H+]i) in single hippocampal neurons by real time fluorescence imaging using the Ca(2+)- and H(+)-specific ratio dyes, indo-1 and SNARF-1. Incubation of these cultures with A beta(25-35) for 3-12 days in vitro increased [Ca2+]i and [H+]i in large, NMDA-responsive neurons.

AB - Insoluble aggregates of the amyloid beta-peptide (A beta) is a major constituent of senile plaques found in brains of Alzheimer disease (AD) patients. The detrimental effects of aggregated A beta is associated with an increased intracellular Ca2+ concentration ([Ca2+]i). We examined the effects of A beta(25-35) on [Ca2+]i and intracellular H+ concentration ([H+]i) in single hippocampal neurons by real time fluorescence imaging using the Ca(2+)- and H(+)-specific ratio dyes, indo-1 and SNARF-1. Incubation of these cultures with A beta(25-35) for 3-12 days in vitro increased [Ca2+]i and [H+]i in large, NMDA-responsive neurons.

M3 - Journal article

C2 - 9631466

SN - 0959-4965

VL - 9

SP - 1553

EP - 1558

JO - NeuroReport

JF - NeuroReport

IS - 7

ER -