AKAP 18 alpha and gamma have opposing effects on insulin release in INS-1E cells

TY - JOUR

T1 - AKAP 18 alpha and gamma have opposing effects on insulin release in INS-1E cells

AU - Josefsen, Knud

AU - Lee, Ying C

AU - Thams, Peter

AU - Efendic, Suad

AU - Nielsen, Jens H

N1 - Keywords: Animals; Carrier Proteins; Cell Line, Tumor; Glucose; Growth Hormone; Insulin; Insulin-Secreting Cells; Rats; Transcription, Genetic

PY - 2010/1/4

Y1 - 2010/1/4

N2 - A-kinase anchoring proteins (AKAPs) are known to compartmentalise protein kinase(s) to discrete cellular locations. Here we show that silencing of AKAP 18 α or γ expression results in decreased or increased glucose-stimulated insulin secretion in INS-1E cells. Glucose stimulates AKAP 18 α and inhibits AKAP 18 γ mRNA expressions while palmitate markedly reduces AKAP 18 α expression. Human growth hormone (GH) stimulates AKAP 18 α expression and attenuates palmitate-induced suppression of AKAP 18 α mRNA level. The roles of AKAP 18 α and γ in mediating insulin release are consistent with their respective regulations by glucose.

AB - A-kinase anchoring proteins (AKAPs) are known to compartmentalise protein kinase(s) to discrete cellular locations. Here we show that silencing of AKAP 18 α or γ expression results in decreased or increased glucose-stimulated insulin secretion in INS-1E cells. Glucose stimulates AKAP 18 α and inhibits AKAP 18 γ mRNA expressions while palmitate markedly reduces AKAP 18 α expression. Human growth hormone (GH) stimulates AKAP 18 α expression and attenuates palmitate-induced suppression of AKAP 18 α mRNA level. The roles of AKAP 18 α and γ in mediating insulin release are consistent with their respective regulations by glucose.

U2 - 10.1016/j.febslet.2009.10.086

DO - 10.1016/j.febslet.2009.10.086

M3 - Journal article

C2 - 19896945

SN - 0014-5793

VL - 584

SP - 81

EP - 85

JO - F E B S Letters

JF - F E B S Letters

IS - 1

ER -