TY - JOUR
T1 - Airway exposure to TiO2 nanoparticles and quartz and effects on sperm counts and testosterone levels in male mice
AU - Lauvås, Anna Jacobsen
AU - Skovmand, Astrid
AU - Poulsen, Marie Sønnegaard
AU - Kyjovska, Zdenka Orabi
AU - Roursgaard, Martin
AU - Goericke-Pesch, Sandra
AU - Vogel, Ulla
AU - Hougaard, Karin Sørig
PY - 2019
Y1 - 2019
N2 - Several types of engineered nanoparticles (ENP) have been shown to adversely affect male reproduction in rodent studies, but the airway route of exposure has been little investigated. This precludes adequate risk assessment of ENP exposure in occupational settings. Titanium dioxide nanoparticles (TiO2 NP) have been shown to affect total sperm count in adult male mice after intravenous and oral administration. This study aimed to investigate whether also airway exposure would affect sperm counts in male mice. Mature C57BL/6J mice were intratracheally instilled with 63 μg of rutile nanosized TiO2, once weekly for seven weeks. Respirable α-quartz (SRM1878a) was included at a similar dose level as a positive control for pulmonary inflammation. BALF cell composition showed neutrophil granulocyte influx as indication of pulmonary inflammation in animals exposed to TiO2 NP and α-quartz, but none of the particle exposures affected weight of testes or the epididymis, sperm counts or plasma testosterone when assessed at termination of the study.
AB - Several types of engineered nanoparticles (ENP) have been shown to adversely affect male reproduction in rodent studies, but the airway route of exposure has been little investigated. This precludes adequate risk assessment of ENP exposure in occupational settings. Titanium dioxide nanoparticles (TiO2 NP) have been shown to affect total sperm count in adult male mice after intravenous and oral administration. This study aimed to investigate whether also airway exposure would affect sperm counts in male mice. Mature C57BL/6J mice were intratracheally instilled with 63 μg of rutile nanosized TiO2, once weekly for seven weeks. Respirable α-quartz (SRM1878a) was included at a similar dose level as a positive control for pulmonary inflammation. BALF cell composition showed neutrophil granulocyte influx as indication of pulmonary inflammation in animals exposed to TiO2 NP and α-quartz, but none of the particle exposures affected weight of testes or the epididymis, sperm counts or plasma testosterone when assessed at termination of the study.
KW - Airways
KW - Inflammation
KW - Intratracheal instillation
KW - Reproduction
KW - Sperm
KW - Testosterone
KW - Titanium dioxide
U2 - 10.1016/j.reprotox.2019.07.023
DO - 10.1016/j.reprotox.2019.07.023
M3 - Journal article
C2 - 31449912
AN - SCOPUS:85072707336
SN - 0890-6238
VL - 90
SP - 134
EP - 140
JO - Reproductive Toxicology
JF - Reproductive Toxicology
ER -