TY - JOUR
T1 - Aerococcus urinae and Aerococcus sanguinicola
T2 - Susceptibility testing of 120 isolates to six antimicrobial agents using disk diffusion (EUCAST), etest, and broth microdilution techniques
AU - Carkaci, Derya
AU - Nielsen, Xiaohui C.
AU - Fuursted, Kurt
AU - Skov, Robert
AU - Skovgaard, Ole
AU - Trallero, Emilio P.
AU - Lienhard, Reto
AU - Åhman, Jenny
AU - Matuschek, Erika
AU - Kahlmeter, Gunnar
AU - Christensen, Jens J.
PY - 2017
Y1 - 2017
N2 - Aerococcus urinae and Aerococcus sanguinicola are relatively newcomers and emerging organisms in clinical and microbiological practice. Both species have worldwide been associated with urinary tract infections. More rarely cases of bacteremia/septicemia and infective endocarditis have been reported. Treatment options are therefore important. Just recently, European recommendations on susceptibility testing and interpretive criteria have been released. Objective: In this investigation 120 A. urinae and A. sanguinicola isolates were tested for susceptibility to six antimicrobial agents: Penicillin, cefotaxime, meropenem, vancomycin, linezolid, and rifampicin. Methods: Three susceptibility testing methods were used; disk diffusion according to The European Committee on Antimicrobial Susceptibility Testing (EUCAST) standardized disk diffusion methodology and MIC determination with Etest and broth microdilution (BMD). All testing was performed with EUCAST media for fastidious organisms. Results: Data obtained in this study were part of the background data for establishing EUCAST breakpoints. MIC values obtained by Etest and BMD were well correlated with disk diffusion results. Conclusion: All isolates were found susceptible to all six antimicrobial agents: penicillin, cefotaxime, meropenem, vancomycin, linezolid, and rifampicin.
AB - Aerococcus urinae and Aerococcus sanguinicola are relatively newcomers and emerging organisms in clinical and microbiological practice. Both species have worldwide been associated with urinary tract infections. More rarely cases of bacteremia/septicemia and infective endocarditis have been reported. Treatment options are therefore important. Just recently, European recommendations on susceptibility testing and interpretive criteria have been released. Objective: In this investigation 120 A. urinae and A. sanguinicola isolates were tested for susceptibility to six antimicrobial agents: Penicillin, cefotaxime, meropenem, vancomycin, linezolid, and rifampicin. Methods: Three susceptibility testing methods were used; disk diffusion according to The European Committee on Antimicrobial Susceptibility Testing (EUCAST) standardized disk diffusion methodology and MIC determination with Etest and broth microdilution (BMD). All testing was performed with EUCAST media for fastidious organisms. Results: Data obtained in this study were part of the background data for establishing EUCAST breakpoints. MIC values obtained by Etest and BMD were well correlated with disk diffusion results. Conclusion: All isolates were found susceptible to all six antimicrobial agents: penicillin, cefotaxime, meropenem, vancomycin, linezolid, and rifampicin.
KW - Aerococcus sanguinicola
KW - Aerococcus urinae
KW - Antimicrobial susceptibility testing
KW - Broth microdilution
KW - Disk diffusion
KW - Etest
KW - Urinary tract infections
U2 - 10.2174/1874285801711010160
DO - 10.2174/1874285801711010160
M3 - Letter
C2 - 29151992
AN - SCOPUS:85032736718
SN - 1874-2858
VL - 11
SP - 160
EP - 166
JO - The Open Microbiology Journal
JF - The Open Microbiology Journal
ER -