TY - JOUR
T1 - Aerobic metabolism of human quadriceps muscle: in vivo data parallel measurements on isolated mitochondria
AU - Rasmussen, Ulla Fugmann
AU - Rasmussen, Hans N.
AU - Krustrup, Peter
AU - Quistorff, B.
AU - Saltin, Bengt
AU - Bangsbo, Jens
N1 - PUF 2001 5200 063
PY - 2001
Y1 - 2001
N2 - The aim of the present study was to examine whether parameters of isolated mitochondria could account for the in vivo maximum oxygen uptake (O2 max) of human skeletal muscle. O2 max and work performance of the quadriceps muscle of six volunteers were measured in the knee extensor model (range 10-18 mmol O2 · min1 · kg1 at work rates of 22-32 W/kg). Mitochondria were isolated from the same muscle at rest. Strong correlations were obtained between O2 max and a number of mitochondrial parameters (mitochondrial protein, cytochrome aa3, citrate synthase, and respiratory activities). The activities of citrate synthase, succinate dehydrogenase, and pyruvate dehydrogenase, measured in isolated mitochondria, corresponded to, respectively, 15, 3, and 1.1 times the rates calculated from O2 max. The respiratory chain activity also appeared sufficient. Fully coupled in vitro respiration, which is limited by the rate of ATP synthesis, could account for, at most, 60% of the O2 max. This might be due to systematic errors or to loose coupling of the mitochondrial respiration under intense exercise. skeletal muscles; maximal oxygen uptake; work rate; respiration; adenosine triphosphate synthesis
AB - The aim of the present study was to examine whether parameters of isolated mitochondria could account for the in vivo maximum oxygen uptake (O2 max) of human skeletal muscle. O2 max and work performance of the quadriceps muscle of six volunteers were measured in the knee extensor model (range 10-18 mmol O2 · min1 · kg1 at work rates of 22-32 W/kg). Mitochondria were isolated from the same muscle at rest. Strong correlations were obtained between O2 max and a number of mitochondrial parameters (mitochondrial protein, cytochrome aa3, citrate synthase, and respiratory activities). The activities of citrate synthase, succinate dehydrogenase, and pyruvate dehydrogenase, measured in isolated mitochondria, corresponded to, respectively, 15, 3, and 1.1 times the rates calculated from O2 max. The respiratory chain activity also appeared sufficient. Fully coupled in vitro respiration, which is limited by the rate of ATP synthesis, could account for, at most, 60% of the O2 max. This might be due to systematic errors or to loose coupling of the mitochondrial respiration under intense exercise. skeletal muscles; maximal oxygen uptake; work rate; respiration; adenosine triphosphate synthesis
M3 - Journal article
SN - 0193-1849
VL - 280
SP - E301-E307
JO - American Journal of Physiology (Endocrinol. Metab.)
JF - American Journal of Physiology (Endocrinol. Metab.)
IS - 2
ER -