Advances in quantitative FRET-based methods for studying nucleic acids

TY - JOUR

T1 - Advances in quantitative FRET-based methods for studying nucleic acids

AU - Preus, Søren

AU - Wilhelmsson, L. Marcus

PY - 2012/9/24

Y1 - 2012/9/24

N2 - Förster resonance energy transfer (FRET) is a powerful tool for monitoring molecular distances and interactions at the nanoscale level. The strong dependence of transfer efficiency on probe separation makes FRET perfectly suited for "on/off" experiments. To use FRET to obtain quantitative distances and three-dimensional structures, however, is more challenging. This review summarises recent studies and technological advances that have improved FRET as a quantitative molecular ruler in nucleic acid systems, both at the ensemble and at the single-molecule levels.

AB - Förster resonance energy transfer (FRET) is a powerful tool for monitoring molecular distances and interactions at the nanoscale level. The strong dependence of transfer efficiency on probe separation makes FRET perfectly suited for "on/off" experiments. To use FRET to obtain quantitative distances and three-dimensional structures, however, is more challenging. This review summarises recent studies and technological advances that have improved FRET as a quantitative molecular ruler in nucleic acid systems, both at the ensemble and at the single-molecule levels.

U2 - 10.1002/cbic.201200400

DO - 10.1002/cbic.201200400

M3 - Journal article

C2 - 22936620

SN - 1439-4227

VL - 13

SP - 1990

EP - 2001

JO - ChemBioChem

JF - ChemBioChem

IS - 14

ER -